For Each of your 3 DNA Templates, Fill out the Following: DNA Template # DNA sequence (copy from the template page) TACGAACTAGTGACCACT Complementary mRNA sequence. Amino Acid Sequence (use the mRNA codon chart above) Protein Number (from the amino acid sequence and protein number page). DNA Template #4 DNA sequence (copy from the template page) TACCCTCGCGCATACATI Complementary mRNA sequence Amino Acid Sequence (use the mRNA codon chart above)_ Protein Number (from the amino acid sequence and protein number page). DNA Template #8 DNA sequence (copy from the template page) TACTCCCTATACCATATO Complementary mRNA sequence Amino Acid Sequence (use the mRNA codon chart above). Protein Number (from the amino acid sequence and protein number page).
Molecular Techniques
Molecular techniques are methods employed in molecular biology, genetics, biochemistry, and biophysics to manipulate and analyze nucleic acids (deoxyribonucleic acid (DNA) and ribonucleic acid (RNA)), protein, and lipids. Techniques in molecular biology are employed to investigate the molecular basis for biological activity. These techniques are used to analyze cellular properties, structures, and chemical reactions, with a focus on how certain molecules regulate cellular reactions and growth.
DNA Fingerprinting and Gel Electrophoresis
The genetic makeup of living organisms is shown by a technique known as DNA fingerprinting. The difference is the satellite region of DNA is shown by this process. Alex Jeffreys has invented the process of DNA fingerprinting in 1985. Any biological samples such as blood, hair, saliva, semen can be used for DNA fingerprinting. DNA fingerprinting is also known as DNA profiling or molecular fingerprinting.
Molecular Markers
A known DNA sequence or gene sequence is present on a chromosome, and it is associated with a specific trait or character. It is mainly used as a genetic marker of the molecular marker. The first genetic map was done in a fruit fly, using genes as the first marker. In two categories, molecular markers are classified, classical marker and a DNA marker. A molecular marker is also known as a genetic marker.
DNA Sequencing
The most important feature of DNA (deoxyribonucleic acid) molecules are nucleotide sequences and the identification of genes and their activities. This the reason why scientists have been working to determine the sequences of pieces of DNA covered under the genomic field. The primary objective of the Human Genome Project was to determine the nucleotide sequence of the entire human nuclear genome. DNA sequencing selectively eliminates the introns leading to only exome sequencing that allows proteins coding.
![mRNA Codon Chart:
Second letter
с
บบบ
UCU
UAU
Phe
UUC
UCC
U
Ser
UAC Tyr
UGU
UGC
Cys
UUA
UCA
Leu
UAA Stop UGA Stop A
UUG
UCG
UAG Stop
UGG Trp
CUU
CCU
CAU
CGU
CUC
CCC
His
CAC
Leu
Pro
CUA
CCA
CAA
CGA
Arg
Gin
CUG
CCG
CAG
CGG
AUU
ACU
AAU
AUC lle
ACC
AAC
AGU
]Asn
Ser
AGC
A
Thr
AUA
ACA
AAA Lys
AGA Arg
AUG Met ACG
AGG
GUU
GCU
GAU
GUC
GCC
Val
GAC Asp
GGU
GGC
Ala
GUA
GCA
GUG
GCG
GAG
GGG
GAA Glu GGA Gly
Amino Acid 3-Letter Codes
Name of Amino
Name of Amino
3-Letter Code
3-Letter Code
Acid
Acid
Alanine
Ala
Leucine
Leu
Arginine
Arg
Lysine
Lys
Asparagine
sex
Methionine
Met (start)
Aspartic Acid
Asp
Phenylalanine
ebe
Cysteine
Cve
Proline
Pro
Glutamic Acid
Glu
Serine
Ser
Glutamine
Gln
Threonine
Glycine
GLK
Tryptophan
JAR
Histidine
His
Tyrosine
Tyr
Isoleucine
lle
Valine
Val
Exercise: Transcription & Translation
1. Choose 3 DNA Templates.
2. Write the mRNA sequence divided into 3 letter codons.
3. Write the sequence of amino acids.
4. Find the corresponding amino acid sequence and include the letter of the corresponding
protein.
For Each of your 3 DNA Templates, Fill out the Following:
DNA Template #
DNA sequence (copy from the template page) TACGAACTAGTGACCACT
Complementary mRNA sequence
Amino Acid Sequence (use the mRNA codon chart above).
Protein Number (from the amino acid sequence and protein number page).
DNA Template #4
DNA sequence (copy from the template page) TACCCTCGCGCATACATI
Complementary mRNA sequence
Amino Acid Sequence (use the mRNA codon chart above).
Protein Number (from the amino acid sequence and protein number page).
DNA Template #8
DNA sequence (copy from the template page)
TACTCCCTATACCATATO
Complementary mRNA sequence
Amino Acid Sequence (use the mRNA codon chart above).
Protein Number (from the amino acid sequence and protein number page).](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fc167378a-6772-4355-bdb0-a5fec1af8d0e%2Fa31dc142-62a5-48e6-afd4-fc4c0184bfea%2Fvq7673x_processed.jpeg&w=3840&q=75)
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