which one is forward primer and which one is reverse primer
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
I want to ask which one is forward primer and which one is reverse primer

Transcribed Image Text:QuikChange II
3. Find your DNA sequence by pressing
Load a file from your hard-drive:
No file selected
4. Load it.
DNA: change
nucleotide(s) to:
or
Upload Now -or- Upload Translated
or specify a DNA region to translate from.
Select up to seven nucleotides that you want to change O
Site 1
Site 2
U1a
agilent.com/store/ +
Insert between two checked nucleotides
10 a
19 g
28 a
37 g
21
11 a
Browse
20 a
29 c
38 t
or
O Delete a region between two checked nucleotides (Note: two checked nucleotides will not be deleted)
c289c_g290a_c291t
3g
12 a
21 a
30 t
39 c
48 t
57 a
66 t
75 g
841
931
46 c
47 t
55 t
56 t
64 g
65 a
73 g
749
82 t
831
91 a
92 g
Finally, obtain your designed primer sequences.
Design Primers
Clear this form to load a new sequence:
Clear Input
Primer sequences:
Primer Name
c289c_g290a_c291t
Oligonucleotide information:
Primer Name
c289c_g290a_c2911
Primer-template duplexes:
Primer Name
Or, paste plain text or FASTA-formatted DNA sequence in the box below:
atggctagcaaaggagaagaacttttcactggagttgtcccaattcttgttgaattaga
tggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgctacat
acggaaagettaccettaaatttatttgcactactggaaaactacctgttccatggcca
acacttgtcactactttctcttatggtgttcaatgcttttcccgttatccggatcatatgaa
acggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactata
tctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgat
accettattaatcatatcoaattaaaaaatattaattttaaaaaaaataaaaacattctc
Length (nt.)
47
47
Primer-Template Duplex
tgecatgeccgaag
3-gtacgE
5-catgeo
|||||
ABOUT AGILENT RESOURCES
acggta
Help in choosing a mutagenesis kit
gtacggget
CONNECT WITH AGILENT f in
49
13 g
22 c
31 g
40 c
49 g
58 g
67 g
76 c
Site 3
851
94 g
▾
Tm
78.59°C
78.59°C
to
L5c
Site 4
V
14 g
23 t
O 32 g
41 c
50 t
59 a
68 t
77 a
86 c
95 g
Primer Sequence (5' to 3)
5-catctttgaaagatatagtatgttcctgtacataaccttcgggcatg-3
5-catgcccgaaggttatgtacaggaacatactatatctttcaaagatg-3
Y
Site 5
AGILENT
SOCIAL HUB
SHOPPING TOOLS SUPPORT &
61
15 a
241
33 a
42 a
51 1
60 t
69t
78 c
87 t
96 a
Duplex Energy at 68 "C
-48.57 kcal/mole
-49.09 kcal/mole
Site 6
7a
16g
251
34 g
43 a
5 :
52 g
61 g
70 a
79 a
88 g
197 g
Upload Translated Region
Site 7
L8g
17 a
26t
35 t
441
53 a
62 g
71 a
80 a
89 t
98 a
aagatgacg
||||||
tttctac-51
tg-31
Y
etge
9c
18
27
36
45
54
63
72
81
Energy Cost of Mismatches
11.31%
12.42%
90
99
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