HDL-R: HDLs carry cholesterol to the liver for elimination. Liver cells respond to estrogen to express a protein receptor for HDLs coded by the HDL-R gene (HDL-R gene contains an estrogen response element.) PFK: codes for an enzyme called phosphofructokinase that is used in the first steps of breaking down glucose for energy in all cells. (Gene has no estrogen response element.) CD3: codes for a cell surface protein produced by immune B cells (Gene has no estrogen response element)mRNA transcripts can be collected from cells, made to glow with a fluorescent dye, and added to a microarray chip containing spots with DNA sequences from each of these genes. (In the depiction of the microarrays below ○ indicates no binding of the mRNA, and ● indicates binding.)Which of the gene expression patterns would you expect to see if you used mRNA from immune B cells to add to the microarray?
Electron Transport Chain
The electron transport chain, also known as the electron transport system, is a group of proteins that transfer electrons through a membrane within mitochondria to create a gradient of protons that drives adenosine triphosphate (ATP)synthesis. The cell uses ATP as an energy source for metabolic processes and cellular functions. ETC involves series of reactions that convert redox energy from NADH (nicotinamide adenine dinucleotide (NAD) + hydrogen (H)) and FADH2(flavin adenine dinucleotide (FAD)) oxidation into proton-motive force(PMF), which is then used to synthesize ATP through conformational changes in the ATP synthase complex, a process known as oxidative phosphorylation.
Metabolism
Picture a campfire. It keeps the body warm on a cold night and provides light. To ensure that the fire keeps burning, fuel needs to be added(pieces of wood in this case). When a small piece is added, the fire burns bright for a bit and then dies down unless more wood is added. But, if too many pieces are placed at a time, the fire escalates and burns for a longer time, without actually burning away all the pieces that have been added. Many of them, especially the larger chunks or damp pieces, remain unburnt.
Cellular Respiration
Cellular respiration is the cellular process involved in the generation of adenosine triphosphate (ATP) molecules from the organic nutritional source obtained from the diet. It is a universal process observed in all types of life forms. The glucose (chemical formula C6H12O6) molecules are the preferred raw material for cell respiration as it possesses a simple structure and is highly efficient in nature.
HDL-R: HDLs carry cholesterol to the liver for elimination. Liver cells respond to estrogen to express a protein receptor for HDLs coded by the HDL-R gene (HDL-R gene contains an estrogen response element.)
PFK: codes for an enzyme called phosphofructokinase that is used in the first steps of breaking down glucose for energy in all cells. (Gene has no estrogen response element.)
CD3: codes for a cell surface protein produced by immune B cells (Gene has no estrogen response element)
mRNA transcripts can be collected from cells, made to glow with a fluorescent dye, and added to a microarray chip containing spots with DNA sequences from each of these genes. (In the depiction of the microarrays below ○ indicates no binding of the mRNA, and ● indicates binding.)
Which of the gene expression patterns would you expect to see if you used mRNA from immune B cells to add to the microarray?
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