John was repeating George Palade's pulse and chase experiment to trace insulin secretion pathway within cells. When he observed his samples under the electron microscope, he saw the³H]-leucine labelling all over the cells, which indicates that he made some mistakes in carrying out the experiment. Which of the following is a correct suggestion to improve his result? O Use liver sample after the pulse-chase injection O Inject [13C]-leucine instead of [3H]-leucine O Inject non-labeled leucine earlier than what he did Use phase contrast light microscope instead of electron microscope Observe the sample two days after pulse-chase injection
John was repeating George Palade's pulse and chase experiment to trace insulin secretion pathway within cells. When he observed his samples under the electron microscope, he saw the³H]-leucine labelling all over the cells, which indicates that he made some mistakes in carrying out the experiment. Which of the following is a correct suggestion to improve his result? O Use liver sample after the pulse-chase injection O Inject [13C]-leucine instead of [3H]-leucine O Inject non-labeled leucine earlier than what he did Use phase contrast light microscope instead of electron microscope Observe the sample two days after pulse-chase injection
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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![John was repeating George Palade's pulse and chase experiment to trace insulin secretion pathway within cells. When he observed his
samples under the electron microscope, he saw the ³H]-leucine labelling all over the cells, which indicates that he made some mistakes
in carrying out the experiment. Which of the following is a correct suggestion to improve his result?
O Use liver sample after the pulse-chase injection
O Inject [13C]-leucine instead of [³H]-leucine
Inject non-labeled leucine earlier than what he did
O Use phase contrast light microscope instead of electron microscope
O Observe the sample two days after pulse-chase injection](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F14c0e5a1-d742-4e41-8d41-3d55fde4a1b7%2Fba83d6b4-18e6-4d23-b5cd-f7a9af995e93%2Ft6xsd3j_processed.jpeg&w=3840&q=75)
Transcribed Image Text:John was repeating George Palade's pulse and chase experiment to trace insulin secretion pathway within cells. When he observed his
samples under the electron microscope, he saw the ³H]-leucine labelling all over the cells, which indicates that he made some mistakes
in carrying out the experiment. Which of the following is a correct suggestion to improve his result?
O Use liver sample after the pulse-chase injection
O Inject [13C]-leucine instead of [³H]-leucine
Inject non-labeled leucine earlier than what he did
O Use phase contrast light microscope instead of electron microscope
O Observe the sample two days after pulse-chase injection
Expert Solution
Step 1
The pulse-chase experiment is a technique used to monitor the progress of newly made proteins within cells. It consists of a short period of labeling the substances used in protein production, followed by a chase with unlabelled substances. This experiment allows us to examine the synthesis, stability, movement, and the breakdown of proteins within cells.
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