Answered: Final State Initial State Ab-Ag Ab Ab…

Final State Initial State Ab-Ag Ab Ab Varying Ag Amounts of Ab-Ag Ab Ab Antigen Ag Ag Ag-Ab-Ag Ab Ab Ab-Ag Ag Ab Ab Ab Ag Membrane permeable to Ag only

Human Physiology: From Cells to Systems (MindTap Course List)

9th Edition

ISBN:9781285866932

Author:Lauralee Sherwood

Publisher:Lauralee Sherwood

Chapter3: The Plasma Membrane And Membrane Potential

Section: Chapter Questions

Problem 5UC

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Question

Biomedical Engineering:

An equilibrium dialysis experiment was performed to characterize the binding affinity of a mouse IgG or human IgG antibody for an antigen, GAD65. (The association constant for the monoclonal mouse IgG is 4.75 x 10^8 M-1 and for a human IgG is 1.3 x 10^10 M-1)

a- calculate the dissociation constant for each antibody and explain which antibody has a higher binding affinity for GAD65?

b-calculate the fraction of free Ab sites for each antibody for a For a GAD65 concentration of 0.7 nM

Final State
Initial State
Ab-Ag
Ab
Ab Varying
Ag
Amounts of
Ab-Ag
Ab Ab
Antigen
Ag
Ag
Ag-Ab-Ag
Ab Ab
Ab-Ag
Ag
Ab
Ab
Ab
Ag
Membrane permeable to Ag only

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