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7. Consider the following plasmid (size 6700 bp), with restriction sites at the positions indicated: BamHI + 1 6700 bp 2800 3500 EcoRI BamHI Probe c) This gel is transferred to a membrane in a Southern blot experiment, and hybridised to a radioactively labelled 100 bp probe, which anneals to the plasmid DNA at the position indicated on the diagram above. Draw the autoradiograph profile you would expect to observe for the membrane, indicate the sizes of the bands on the blot.

7. Consider the following plasmid (size 6700 bp), with restriction sites at the positions indicated: BamHI + 1 6700 bp 2800 3500 EcoRI BamHI Probe c) This gel is transferred to a membrane in a Southern blot experiment, and hybridised to a radioactively labelled 100 bp probe, which anneals to the plasmid DNA at the position indicated on the diagram above. Draw the autoradiograph profile you would expect to observe for the membrane, indicate the sizes of the bands on the blot.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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7. Consider the following plasmid (size 6700 bp), with restriction sites at the positions indicated: BamHI + 1 6700 bp 2800 3500 EcoRI BamHI Probe
Transcribed Image Text:7. Consider the following plasmid (size 6700 bp), with restriction sites at the positions indicated: BamHI + 1 6700 bp 2800 3500 EcoRI BamHI Probe
c) This gel is transferred to a membrane in a Southern blot experiment, and hybridised to a radioactively labelled 100 bp probe, which anneals to the plasmid DNA at the position indicated on the diagram above. Draw the autoradiograph profile you would expect to observe for the membrane, indicate the sizes of the bands on the blot.
Transcribed Image Text:c) This gel is transferred to a membrane in a Southern blot experiment, and hybridised to a radioactively labelled 100 bp probe, which anneals to the plasmid DNA at the position indicated on the diagram above. Draw the autoradiograph profile you would expect to observe for the membrane, indicate the sizes of the bands on the blot.
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