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- what are the volume dilution and dilution factor for bacteriophage A,B,CShown below is a set of cell culture plates for a plaque assay. The assay was performed by preparing dilutions of a virus stock with the dilution factor for each prepared dilution listed below the sample. 0.5 mls of each dilution was added to confluent (fully covered) monolayers of cells on each plate. The virus used for the assay had a titer of 2 x 1010 Plaque forming units (PFU) per ml. Only 1 set of the above plates would have a number of plaques on it that would be both easy to count and high enough to be statistically relevant. Which dilution set would it be and about how many plaques would there be on the plates of that dilution set.A serial dilution of overnight E.coli culture was performed by pipetting 1ml of a bacterial culture into a 9 ml LB medium. After this, from 10-4 and 10-5 dilution tubes 100µl were plated onto LB agar plates. Upon overnight incubation at 37°C, 200 colonies were counted in 10-4 and 22 colonies were present on 10-5 plates. How many colony-forming units were present per ml of the original culture? If the formula for CFU/ml =no. Of colonies/dilution factor*volume of culture plate
- Average plaques for bacteriophage A,B,C are 137,36,25. PFU/ml is average plaques multiply by the volume dilution multiply by the dilution factor. Show your working for each bacteriophage.____________ help to transfer DNA between bacterial cells during the conjugation.You have several different media onto which you inoculated eight strains of yeast (A-H). The media include a rich medium, an unsupplemented minimal medium, and minimal media each supplemented with one vitamin. Of the yeast strains, one is a prototroph and seven are auxotrophs for a vitamin. After overnight incubation, the following results were observed (tan patches represent growth): D plate 1 (A) B DE F GH plate 5 plate 4 plate 6 Which plate contains an unsupplemented minimal medium? [Select] Which plate contains a rich medium? [Select] plate 2 Which strain is a prototroph? [Select] Strain E is an auxotroph for niacin. Which plate reveals this specific auxotrophy? [ Select] plate 3 plate 7 One strain is an auxotroph for both choline and pantothenic acid. Which one is this most likely to be? [Select]
- Three different strains of Escherichia coli, respectively A, B, and C have been inoculated in three different media, namely I, II, and III, and the growth of each bacterial strain has been followed by spectrophotometry (Figure 1). Strain A Strain B Strain C 4.5 45 Medium I 4 3.5 25 2.5 3 E 2.5 Minimal medium + Glucose 25 2.5 O 15 1 0.5 as 05 100 200 300 400 500 600 100 200 400 600 200 Time (minj Strain A Strain B StrainB 4.5 4s 4s Medium II 3.5 15 25 2 3 Minimal 25 2 medium + Glucose 1 + Arginine 0.5 as as 100 200 300 400 500 600 100 300 400 s00 600 100 300 400 600 Tme Time (min) Strain A me StreinB Streine 4.5 45 45 Medium II 4 4. 3.5 3.5 25 2 3 Minimal medium + Glucose 5 2.5 25 25 8 15 15 + Lactose 1 0.5 05 05 100 200 300 400 500 600 300 200 400 10 200 300 630 Time (min) Tine imin Time min Figure 1. Growth curves of the three E. coli strains A, B and C in three media: I, minimal medium containing glucose; II, minimal medium containing glucose and arginine; and III, minimal medium…Write a paragraph for when you once have Neisseria gonorrhoea bacteria in its pure form, what methods can you use to confirm the identity of this bacterium as N. gonorrhoeae? Mention what is the principle of those methods.Table below shows the results from transduction experiment. The experiment was completed in duplicate. Calculate the average number of transductants resulting from this experiment (show your calculations). List 3 reasons for the bacterial growth on the control plates. Culture Transduction Minimal Medium E.coli control 1 5 E.coli control 2 3 E.coli + P-22 plate 1 37 E.coli + P-22 plate 2 41
- I completed the chart, and I'm supposed to figure out the unknown bacteria, there are 12 unknown culture, out of which of them resemble my chart the most? (My chart is the one in pink with the information filled out)TRY TO KEEP IN SHORT AND USE OWN WORD FOR THIS QUESTION You are studying a type of bacteria isolated from the acidic water runoff of a mining operation. You subject two batches of the same bacteria type to different environmental growth conditions. One batch is grown at pH 2, while the other is grown at pH 7. All other environmental parameters are kept identical between the two batches. You then collect their proteins and run a Western blot using an antibody that binds to a proton efflux pump protein (which actively expends energy to pump protons out of a cell). How would you characterize the information obtained in this experiment? What does it tell you, and why is that potentially valuable information?Write a short discussion based on the result