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- How does the microscopy in Figure 2 show that the capsule and an S-layer can exist in the same cell at the same time? I need help finding the answer in the article and explain in short answer link to article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC106848/The following pictures show the results of a Disk Diffusion Assay for different types of bacteria. For each bacteria, what antibiotic would you recommend be used on the patient? Explain your choice.Escherichia coli Mycobacterium phlei Bacillus Micrococcus subtilis luteus A B Figure 1: Agar Slant Cultures of Bacteria (Gary E. Kaiser, Ph.D.- Professor of Microbiology) . Observe and describe the colour of the slant cultures (A-D) in fig 1. ( . Define the following terms: pure culture, sterile medium, inoculum, aseptic technique, and colony. What is the name of the cultures that you used . Where they gram negative or positive Define the following terms: psychrophile, mesophile, thermophile, and hyperthermophile.
- what are the volume dilution and dilution factor for bacteriophage A,B,CShown below is a set of cell culture plates for a plaque assay. The assay was performed by preparing dilutions of a virus stock with the dilution factor for each prepared dilution listed below the sample. 0.5 mls of each dilution was added to confluent (fully covered) monolayers of cells on each plate. The virus used for the assay had a titer of 2 x 1010 Plaque forming units (PFU) per ml. Only 1 set of the above plates would have a number of plaques on it that would be both easy to count and high enough to be statistically relevant. Which dilution set would it be and about how many plaques would there be on the plates of that dilution set.Hello, please read the attached Microbiology question and answer both parts correctly. Please explain your answer. *If you correctly answer the question and its two parts, I will provide a Thumbs Up to you. Thank you.
- Suppose your professor handed you a test tube with 2.0 mL of M. foliorum infected with bacteriophage T4 in it and told you to make a 10-2 dilution of the entire culture. Explain how you would do this. Show your calculations.You have several different media onto which you inoculated eight strains of yeast (A-H). The media include a rich medium, an unsupplemented minimal medium, and minimal media each supplemented with one vitamin. Of the yeast strains, one is a prototroph and seven are auxotrophs for a vitamin. After overnight incubation, the following results were observed (tan patches represent growth): D plate 1 (A) B DE F GH plate 5 plate 4 plate 6 Which plate contains an unsupplemented minimal medium? [Select] Which plate contains a rich medium? [Select] plate 2 Which strain is a prototroph? [Select] Strain E is an auxotroph for niacin. Which plate reveals this specific auxotrophy? [ Select] plate 3 plate 7 One strain is an auxotroph for both choline and pantothenic acid. Which one is this most likely to be? [Select]Write a paragraph for when you once have Neisseria gonorrhoea bacteria in its pure form, what methods can you use to confirm the identity of this bacterium as N. gonorrhoeae? Mention what is the principle of those methods.
- Table below shows the results from transduction experiment. The experiment was completed in duplicate. Calculate the average number of transductants resulting from this experiment (show your calculations). List 3 reasons for the bacterial growth on the control plates. Culture Transduction Minimal Medium E.coli control 1 5 E.coli control 2 3 E.coli + P-22 plate 1 37 E.coli + P-22 plate 2 41TRY TO KEEP IN SHORT AND USE OWN WORD FOR THIS QUESTION You are studying a type of bacteria isolated from the acidic water runoff of a mining operation. You subject two batches of the same bacteria type to different environmental growth conditions. One batch is grown at pH 2, while the other is grown at pH 7. All other environmental parameters are kept identical between the two batches. You then collect their proteins and run a Western blot using an antibody that binds to a proton efflux pump protein (which actively expends energy to pump protons out of a cell). How would you characterize the information obtained in this experiment? What does it tell you, and why is that potentially valuable information?In a petri dish with solidified agar with escherichia coli, enterobacter aerogenes and staphylococcus aureus, you streak a loopfull of lytic T4-phage in a single line onto the center of the the dish, how do you know if bacteriophage infected the bacteria. why didn't the bacteriophage infect all 3 bacteria?