DNA ladder Sample digested with EcoRI negative electrode 23- This is the result of gel electrophoresis of the unknown DNA 8000 bp (base pairs) sample digested with EcoRI. The size of three fragments are indicated in the result (8000 bp, 5800 bp, and 850 bp). 5800 bp (1) How many ECORI restriction enzyme cut site are available 2.3 in the unknown DNA? (Assume that the DNA was fully 850 bp digested with EcoRI) positive electrode slab of agarose (2) What is the size (bp) of the unknown DNA? (A) nucleotide pairs (x 1000)

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Genetic Recombination

Recombination is crucial to this process because it allows genes to be reassorted into diverse combinations. Genetic recombination is the process of combining genetic components from two different origins into a single unit. In prokaryotes, genetic recombination takes place by the unilateral transfer of deoxyribonucleic acid. It includes transduction, transformation, and conjugation. The genetic exchange occurring between homologous deoxyribonucleic acid sequences (DNA) from two different sources is termed general recombination. For this to happen, an identical sequence of the two recombining molecules is required. The process of genetic exchange which occurs in eukaryotes during sexual reproduction such as meiosis is an example of this type of genetic recombination.

Microbial Genetics

Genes are the functional units of heredity. They transfer characteristic information from parents to the offspring.