Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
Related questions
Question
3. Solve the sequence of an oligopeptide 7 residues long which gave:
Asp Leu Lys Met Phe Tyr
The following facts were observed:
a. Trypsin treatment had no apparent effect
b. The PTH derivative from Edman degradation was
c. Brief chymotrypsin treatment yielded several products including but not limited to a dipeptide and a tetrapeptide. The amino acid composition of the tetrapeptide was Leu, Lys, and Met.
d. Cyanogen bromide treatment yielded a dipeptide, a tetrapeptide, and a free Lys.
Instructions
Make use of the table below to determine the sequence of the mystery protein.

Transcribed Image Text:Reagent
Cleavage Points
Trypsin
Lys, Arg (C)
Submaxillary protease
Arg (C)
Chymotrypsin
Phe, Trp, Tyr (C)
2/4
Staphylococcus aureus
Asp, Glu (C)
Asp-N-protease
Asp, Glu (N)
Pepsin
Leu, Phe, Trp, Tyr (N)
Endoproteinase Lys C
Lys (C)
Cyanogen bromide
Met (C)
Carboxypeptidase A
Works on all residues (C) except Pro, Arg, Lys,
Asp, and Glu
Carboxypeptidase B
Arg, Lys (C)
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