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With the ninhydrin method, it was determined that an acyclic decapeptide consists of the following amino acids: Ala, Arg, Gly, Leu, Phe, Ser, Tyr, Val. Find the primary structure of this peptide, if the following are true: a) Treatment with Edman's reagent gave PTH-Gly. b) Effect of carboxypeptidase A did not yield any amino acid. c) With the effect of elastase, 3 oligopeptides were obtained which, by the ninhydrin method, were found to contain the following amino acids: C1: Ala, Arg, Ser, Tyr, Val C2: Arg, Leu, Phe C3: Gly d) With the effect of chymotrypsin, 3 oligopeptides were obtained which, with the ninhydrin method, were found to contain the following amino acids: D1: Ala, Phe, Ser D2: Arg, Gly, Tyr, Val D3: Arg, Leu e) With the effect of trypsin, 2 oligopeptides were obtained which, with the ninhydrin method, were found to contain the following amino acids: E1: Ala, Arg, Leu, Phe, Ser, Tyr E2: Arg, Gly, Val Hint: C3 is a dipeptide.

With the ninhydrin method, it was determined that an acyclic decapeptide consists of the following amino acids: Ala, Arg, Gly, Leu, Phe, Ser, Tyr, Val. Find the primary structure of this peptide, if the following are true: a) Treatment with Edman's reagent gave PTH-Gly. b) Effect of carboxypeptidase A did not yield any amino acid. c) With the effect of elastase, 3 oligopeptides were obtained which, by the ninhydrin method, were found to contain the following amino acids: C1: Ala, Arg, Ser, Tyr, Val C2: Arg, Leu, Phe C3: Gly d) With the effect of chymotrypsin, 3 oligopeptides were obtained which, with the ninhydrin method, were found to contain the following amino acids: D1: Ala, Phe, Ser D2: Arg, Gly, Tyr, Val D3: Arg, Leu e) With the effect of trypsin, 2 oligopeptides were obtained which, with the ninhydrin method, were found to contain the following amino acids: E1: Ala, Arg, Leu, Phe, Ser, Tyr E2: Arg, Gly, Val Hint: C3 is a dipeptide.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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8 With the ninhydrin method, it was determined that an acyclic decapeptide consists of the following amino acids: Ala, Arg, Gly, Leu, Phe, Ser, Tyr, Val. Find the primary structure of this peptide, if the following are true: a) Treatment with Edman's reagent gave PTH-Gly. b) Effect of carboxypeptidase A did not yield any amino acid. c) With the effect of elastase, 3 oligopeptides were obtained which, by the ninhydrin method, were found to contain the following amino acids: C1: Ala, Arg, Ser, Tyr, Val C2: Arg, Leu, Phe C3: Gly d) with the effect of chymotrypsin, 3 oligopeptides were obtained which, with the ninhydrin method, were found to contain the following amino acids: D1: Ala, Phe, Ser D2: Arg, Gly, Tyr, Val D3: Arg, Leu e) With the effect of trypsin, 2 oligopeptides were obtained which, with the ninhydrin method, were found to contain the following amino acids: E1: Ala, Arg, Leu, Phe, Ser, Tyr E2: Arg, Gly, Val Hint: C3 is a dipeptide.
Transcribed Image Text:8 With the ninhydrin method, it was determined that an acyclic decapeptide consists of the following amino acids: Ala, Arg, Gly, Leu, Phe, Ser, Tyr, Val. Find the primary structure of this peptide, if the following are true: a) Treatment with Edman's reagent gave PTH-Gly. b) Effect of carboxypeptidase A did not yield any amino acid. c) With the effect of elastase, 3 oligopeptides were obtained which, by the ninhydrin method, were found to contain the following amino acids: C1: Ala, Arg, Ser, Tyr, Val C2: Arg, Leu, Phe C3: Gly d) with the effect of chymotrypsin, 3 oligopeptides were obtained which, with the ninhydrin method, were found to contain the following amino acids: D1: Ala, Phe, Ser D2: Arg, Gly, Tyr, Val D3: Arg, Leu e) With the effect of trypsin, 2 oligopeptides were obtained which, with the ninhydrin method, were found to contain the following amino acids: E1: Ala, Arg, Leu, Phe, Ser, Tyr E2: Arg, Gly, Val Hint: C3 is a dipeptide.
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