3. Determine the primary structure of pentadecapeptide X, based on the following results. A). Reagent: merchapthoethanol Released two fragments, namely: 1. pentapeptide (Y), amino acid composition: cys, phe, ser, thr, val 2. decapeptide (Z), amino acid composition: ala(2), cys, leu, lys, phe, pro, tyr, B). Process: Edman Degradation 1. Nt of peptide Y is ser, 2. Nt of peptide Z is ala. C). Enzyme: Carboxypeptidase A 1. val was released from peptide Y; 2. no amino acid was released from peptide Z D). Enzyme: Chymotrypsin 1. peptide Y released two fragments with the following amino acid composition. (phe, ser, thr) (cys, val) 2. peptide Z released three fragments with the following amino acid composition (ala [2], tyr) val(2) 10 (cys, phe) (leu, lys, val, pro, val) E). Enzyme: trypsin peptide Z released two fragments with the following amino acid composition ala (2), cys, leu, lys, phe, tyr val (2), pro
3. Determine the primary structure of pentadecapeptide X, based on the following results. A). Reagent: merchapthoethanol Released two fragments, namely: 1. pentapeptide (Y), amino acid composition: cys, phe, ser, thr, val 2. decapeptide (Z), amino acid composition: ala(2), cys, leu, lys, phe, pro, tyr, B). Process: Edman Degradation 1. Nt of peptide Y is ser, 2. Nt of peptide Z is ala. C). Enzyme: Carboxypeptidase A 1. val was released from peptide Y; 2. no amino acid was released from peptide Z D). Enzyme: Chymotrypsin 1. peptide Y released two fragments with the following amino acid composition. (phe, ser, thr) (cys, val) 2. peptide Z released three fragments with the following amino acid composition (ala [2], tyr) val(2) 10 (cys, phe) (leu, lys, val, pro, val) E). Enzyme: trypsin peptide Z released two fragments with the following amino acid composition ala (2), cys, leu, lys, phe, tyr val (2), pro
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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