Determine the primary structure of pentadecapeptide X, based on the following results.A). Reagent: merchapthoethanolReleased two fragments, namely:1. pentapeptide (Y), amino acid composition: cys, phe, ser, thr, val2. decapeptide (Z), amino acid composition: ala(2), cys, leu, lys, phe, pro, tyr,B). Process: Edman Degradation1. Nt of peptide Y is ser, 2. Nt of peptide Z is ala.C). Enzyme: Carboxypeptidase A1. val was released from peptide Y; 2. no amino acid was released from peptide ZD). Enzyme: Chymotrypsin1. peptide Y released two fragments with the following amino acid composition.(phe, ser, thr)(cys, val)2. peptide Z released three fragments with the following amino acid composition3.val(2)(ala [2], tyr)10(cys, phe)(leu, lys, val, pro, val)E). Enzyme: trypsinpeptide Z released two fragments with the following amino acid compositionala (2), cys, leu, lys, phe, tyrval (2), pro

Determination of protein primary structure is done by degradation with different enzymes which have…

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Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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25) Below are two amino acids: cysteine and histidine. a) Draw the reaction mechanism of a dehydration synthesis reaction between cysteine and histidine below: HS H Product 1 N OH HN H b) Draw two different products of the dehydration synthesis reaction from part a). Prove that these are two different products by labelling the N and C termini, peptide bond, and R-groups of each product. Product 2 OH
1. Label the ALPHA-Carbon, AMINO Group, CARBOXYL Group, and AMINO Side Chain of the following amino acids:- Proline- Glycine- Aspartic acid- Asparagine- Lysine 2. Write the structure of the following polypeptide chainsa.) Asp-Leu-Serb.) Thr-Trpc.) Gln-Ala-Prod.) Ile-Met-Cys-Glu
9) Propose a synthetic sequence for the dipeptide A-D-G (single-letter codes) starting from the free amino acids. Draw reaction equations with structural formulas of all organic reagents for the individual steps. Which protecting strategy of the side-chain functional group is appropriate? Please draw the mechanism of peptide bond formation.
Which of the following statements about aromatic amino acids is correct? (a) All are strongly hydrophilic. (b) Histidine's ring structure results in its being categorized as aromatic or basic, depending on pH. (c) On a molar basis, tryptophan absorbs more ultraviolet light than tyrosine. (d) The major contribution to the characteristic absorption of light at 280 nm by proteins is the phenylalanine R group. (e) The presence of a ring structure in its R group determines whether or not an amino acid is aromatic.
What are the structural differences between bilirubin and urobilin? (Select all that apply.) O Ring is opened in the first compound, but is not in the second compound. Several methylene bridges are reduced in the second compound. O Iron atom is absent in the second compound, but is present in the first compound. The central methylene bridge is reduced in the second compound, but is not in the first compound. O Ring is opened in the second compound, but is not in the first compound. Some vinyl groups are reduced into ethyl groups in the second compound. The central methylene bridge is oxidised in the second compound, but is not in the first compound. O Some vinyl groups are reduced into ethyl groups in the first compound.
2) Draw the skeletal (line-boad) structure for 2-methylbutanediaicacid. 3) Draw the skeletal structuce for (E)-3-phenylbut - 2-cnois acid. 4) Draw the skele tal structure for (2)-3-chloro-2- phenylprop-2-enaic acid. 5) The common fea ture of all esters is the presence of a A)-OH group B)-CE8)OH group C)-C(=0)H group D)-C(=0)OR group. 6) Draw the skeletal structuce of 3,3-dimethylbutanenitrile
The urine of an infant gives a positive reaction with 2,4 dinitrophenylhydrazine. Mass spectrometry shows abnormally high blood levels of pyruvate, alpha-ketoglutarate, and the alpha-ketoacids of valine, isoleucine, leucine and threonine. Identify a likely molecular defect and propose a definitive test of your diagnosis.
2) Consider the following disaccharide which was formed from the breakdown of a polysaccharide found in plant material. ОН Glycosidic linkage: H H OH H H НО H H. OH H. ОН OH H НО H ОН a. Label the glycosidic linkage above in the following format: a/B(# → #) b. Would you expect this disaccharide to have been formed from amylose or amylopectin? c. Describe at least two chemical tests that would give insight into the structure of this disaccharide, assuming you've isolated it and wanted to elucidate the structure from scratch. Include detail on not only the tests you would use but what results you would expect to get given the structure above. Assume you can analyze the structure of any monosaccharide you get from your chemical tests.
Determine the sequence of the following 10-mer of ferritin using the data below. i. Complete acid hydrolysis of the 10-mer results in identification of 1A, 1D, 1E, 1F, 1H, 1K, 2L, 1T, 1Y ii. Digestion of the 10-mer with chymotrypsin results in 3 fragments containing (D, H, K, T), (F, L) and (A, E, L, Y) iii. Digestion of the 10-mer with V8 protease, which cuts on the C-side of acidic amino acids, results in three fragments containing (D, F, L, Y), (A, E, L) and (H, K, T) iv. Digestion with trypsin results in a fragment H-T and a fragment with all the other amino acids v. Treatment of the 10-mer with FDNB followed by complete acid hydrolysis results in the following two derivatives, DNP-1 and DNP-2 vi. Treatment of the 10-mer with a carboxypeptidase results in a free threonine
1) Indicate the total charge of the peptide at pH 7.0, 9.0, and 11.0 2) Determine the isoelectric point (pI) for this peptide. Show the relevant pKa’s and your calculation. 3) What is the fraction of the peptide that has the N-terminal amino group deprotonated at pH 9.
47. Tretinoin gel (RETIN-A MICRO) is avail- able in two strengths: 0.1% w/w and 0.04% w/w. Which of these represents equivalent concentrations of the two strengths? (a) 15 mg/15 g and 30 mg/30 g (b) 5 mg/5 g and 0.06 g/15 g (c) 15 mg/15 g and 60 mg/15 g (d) 30 mg/30 g and 6 mg/15 g 47. (d) 30 mg/30 g and 6 mg/15 g
16.B. The formula for "Lubow's Solution" is as follows3: 1 g 500 mg Progesterone Hydrocortisone 2 g Propylene glycol Ethanol (95%) ad 100 mL (a) Calculate the quantities of each ingredient required to prepare 2.5 mL of solution. (b) How many milliliters of propylene glycol (sp. gr. 1.04) are needed to prepare a liter of the formula?

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