QUESTION 1Determine the OPTIMUM pH for the enzyme reaction using the software simulation:(HINT: Run the enzyme reaction at the OPTIMUM temperature for the most accurate results)02 03 04 05 06 07 08 09 10 11 12QUESTION 2Determine the OPTIMUM temperature for the enzyme reaction using the software simulation:(HINT: Run the enzyme reaction at the OPTIMUM pH for the most accurate results)O 27 °C 28 °C 29 °C O OO 33 34 OOO 43 44 45°CQUESTION 3Calculate the Km of the enzyme without any inhibitor when using 6U of Alkaline phosphatase per reaction under the OPTIMAL conditions for pH andtemperature to obtain the raw data.(In mM)ㅎㅇOOC

Enzymes are the biocatalyst which increases the rate of reaction without itself being changed in…

Answered: Determine the OPTIMUM pH for the enzyme…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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A biochemist wants to determine the effect of an inhibitor on a certain enzyme. The data are shown below: Vo (mM/min) (without inhibitor) Vo (mM/min) (with inhibitor) [Substrate] (mM) 15 1.11 0.67 30 1.81 1.17 45 2.28 1.56 60 2.62 1.88 90 3.09 2.36 Using linear regression analysis, determine the values of Vmax and Km of the enzyme in the ab- sence of an inhibitor. - y-int = - slope = - vmax = - Km =
Before Lab: Compare the structures below to the structure of the substrate. Which do you think would be the most effective inhibitor of alkaline phosphatase? Which do you think would be the least effective inhibitor of alkaline phosphatase? glyphosate citric acid MES buffer cyclamate cantharidin HN HO HO, H-N OH H H- 7
A continuous enzyme reactor consists of a stirred tank of volume 0.01 m3 through which liquid is pumped at 0.02 m3⁄h. The enzyme is immobilised onto non-porous, spherical support particles, and the contents are stirred well to ensure that the composition is uniform and that the particles remain in suspension. A plastic mesh has been placed in front of the exit pipe to prevent the particles from leaving the vessel. The reactor is used to carried out a 98% conversion of a substrate entering at 0.1 kmol m3 ⁄ . Michaelis-Menten kinetics apply, with the following enzyme characteristics: Turnover number = 1 × 10^3 s KM = 5 × 10−3 kmol m3 ⁄ Enzyme RMM = 65,000 (a) If the enzyme is stable, calculate the mass of enzyme that needs to be immobilised. (b) If the enzyme is not stable and experiences exponential denaturation with a half-life of 10 h at the reaction temperature, determine the denaturation constant. (c) How would you expect denaturation to affect the reactor performance?
You run a series of assays at 25°C on enzyme A. You measure the velocity for a range of S concentrations. What is the Km (in mM) for Enzyme A?
If the higher value of KM resulting in the new plot ( red curb ) is due to the presence of an enzyme inhibitor is inhibitor reversible or irreversible? And why?
Please show all work/answers for the question
Background information: A static respirometer can be used to measure metabolic rates of small aquatic organisms such as fish and crayfish. The animal is weighed and placed in the chamber. The chamber is then filled completely with water and sealed, and the dissolved oxygen level in the water is measured for 30 – 60 minutes. The animal is then removed and the respirometer is rinsed before being filled with clean water for the next trial. Metabolic rates can be calculated knowing the oxygen consumption rate, the volume of the chamber, and the weight of the animal. The picture shows a typical static respirometer. The probes on the lid measure dissolved oxygen and temperature and are linked to a computer for data collection. When using a static respirometer, the usual procedure calls for filling the chamber completely with water and making sure there is no air left at the top of the chamber when it is sealed. Why is this important, i.e., how would trapped air influence the metabolic…
You put 50 uL of 0.1 mg of protein into a 1 cm pathlength cuvette containing 3 mL of LDH reaction mixture and measure a slope of 0.125 abs/min in an LDH assay. What is the specific activity of the enzyme sample? Show your work.
What is the parameter in the enzyme measurements?
Why is it important to know the Km and Vmax values of an enzyme?
| 2 3 I 4 I 5 I 6 I 7 Directions: Graph the data (line graph); label both axes. DATA TABLE 1 Temperature (°C) Enzyme Activity 10 10 20 15 30 25 40 45 50 35 60 20 Temperature (C) 1. According to the graph, what is the best (optimum) temperature for enzyme activity? Explain your reasoning. 2. What happens to the enzyme above the optimum temperature? 26°F F12 PrtSc Insert Delete F7 FO F9 Backspac 7 8. R P G K 近

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Determine the OPTIMUM pH for the enzyme reaction using the software simulation: (HINT: Run the enzyme reaction at the OPTIMUM temperature for the most accurate results) 2 03 04 05 06 07 08 09 10 11 12