When using a static respirometer, the usual procedure calls for filling the chamber completely with water and making sure there is no air left at the top of the chamber when it is sealed.  Why is this important, i.e., how would trapped air influence the metabolic rate estimates?

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Background information: A static respirometer can be used to measure metabolic rates of small aquatic organisms such as fish and crayfish.  The animal is weighed and placed in the chamber.  The chamber is then filled completely with water and sealed, and the dissolved oxygen level in the water is measured for 30 – 60 minutes. The animal is then removed and the respirometer is rinsed before being filled with clean water for the next trial.  Metabolic rates can be calculated knowing the oxygen consumption rate, the volume of the chamber, and the weight of the animal. The picture shows a typical static respirometer.  The probes on the lid measure dissolved oxygen and temperature and are linked to a computer for data collection.



When using a static respirometer, the usual procedure calls for filling the chamber completely with water and making sure there is no air left at the top of the chamber when it is sealed.  Why is this important, i.e., how would trapped air influence the metabolic rate estimates?

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