An Hfr+ strain is used to map three genes: ala, leu, and thr in an interrupted mating experiment. Hfr/ala+, leu+, thr+, camS x F-/ala-, leu-, thr-, camR The cross is initiated at time = 0, and at various times, the mating mixture is plated on three types of medium. Each plate contains minimal medium with chloramphenicol and the amino acids as listed in the table. The data represents the number of colonies growing on each plate. Time of interruption Nutrients added 5 min 10 min 15 min 20 min alanine, leucine 8 21 58 85 leucine, threonine 0 3 19 46 alanine, threonine 0 0 7 16 Based on these data, what is the order of gene transfer relative to one another and to the F-factor.
. An Hfr+ strain is used to map three genes: ala, leu, and thr in an interrupted mating experiment.
Hfr/ala+, leu+, thr+, camS x F-/ala-, leu-, thr-, camR
The cross is initiated at time = 0, and at various times, the mating mixture is plated on three types of medium. Each plate contains minimal medium with chloramphenicol and the amino acids as listed in the table. The data represents the number of colonies growing on each plate.
Time of interruption |
||||
Nutrients added |
5 min |
10 min |
15 min |
20 min |
alanine, leucine |
8 |
21 |
58 |
85 |
leucine, threonine |
0 |
3 |
19 |
46 |
alanine, threonine |
0 |
0 |
7 |
16 |
Based on these data, what is the order of gene transfer relative to one another and to the F-factor.
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