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You have isolated several loss-of-function com mutations that prevent bacterial cells from growing on the disaccharide sugar "comatose". The mutations map to two linked but separate sequences, comA and comB. You have found that comA encodes an enzyme required for comatose catabolism. You construct two F'strains that are merodiploid for both comA and comB, and test their ability to grow on comatose. The results are: comA- comB/F' comA+ comB+ comA+ comB-/F' comA- comB+ grows on comatose. does not grow on comatose. strain 1: strain 2: No order is implied by the genotypes. What is the wild type comB locus most likely to be or to gene encode? a cis-acting site for positive regulation. a cis-acting site for negative regulation. a trans-acting positive regulator. a trans-acting negative regulator. a second enzyme required for comatose catabolism.

You have isolated several loss-of-function com mutations that prevent bacterial cells from growing on the disaccharide sugar "comatose". The mutations map to two linked but separate sequences, comA and comB. You have found that comA encodes an enzyme required for comatose catabolism. You construct two F'strains that are merodiploid for both comA and comB, and test their ability to grow on comatose. The results are: comA- comB/F' comA+ comB+ comA+ comB-/F' comA- comB+ grows on comatose. does not grow on comatose. strain 1: strain 2: No order is implied by the genotypes. What is the wild type comB locus most likely to be or to gene encode? a cis-acting site for positive regulation. a cis-acting site for negative regulation. a trans-acting positive regulator. a trans-acting negative regulator. a second enzyme required for comatose catabolism.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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You have isolated several loss-of-function com mutations that prevent bacterial cells from growing on the disaccharide sugar "comatose". The mutations map to two linked but separate sequences, comA and comB. You have found that comA encodes an enzyme required for comatose catabolism. You construct two F'strains that are merodiploid for both comA and comB, and test their ability to grow on comatose. The results are: comA- comB/F' comA+ comB+ comA+ comB-/F' comA- comB+ grows on comatose. does not grow on comatose. strain 1: strain 2: No order is implied by the genotypes. What is the wild type comB locus most likely to be or to gene encode? a cis-acting site for positive regulation. a cis-acting site for negative regulation. a trans-acting positive regulator. a trans-acting negative regulator. a second enzyme required for comatose catabolism.
Transcribed Image Text:You have isolated several loss-of-function com mutations that prevent bacterial cells from growing on the disaccharide sugar "comatose". The mutations map to two linked but separate sequences, comA and comB. You have found that comA encodes an enzyme required for comatose catabolism. You construct two F'strains that are merodiploid for both comA and comB, and test their ability to grow on comatose. The results are: comA- comB/F' comA+ comB+ comA+ comB-/F' comA- comB+ grows on comatose. does not grow on comatose. strain 1: strain 2: No order is implied by the genotypes. What is the wild type comB locus most likely to be or to gene encode? a cis-acting site for positive regulation. a cis-acting site for negative regulation. a trans-acting positive regulator. a trans-acting negative regulator. a second enzyme required for comatose catabolism.
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