An Hfr strain that is lacZ" and galE" was mated to a strain that is lacZ and galE. The matingwas interrupted and the percentage of recombinants for each gene was determined bystreaking on plates that contain either lactose or galactose as the sole carbon source. Thefollowing results were obtained:30-lacz2010-galE011016 20 25 304050Duration of mating (minutes)(i)Determine the map distance (in minutes) between these two genes.In a previous experiment, it was found that lacZ is 6 minutes away from gene hisB.galE was shown to be 15 minutes from this gene. Draw a genetic map describing thelocations of all three genes.(ii)% of F recipient cellsthat have received thegene during conjugation

Introduction: The transfer of the genetic material from one bacterium to another via the conjugation…

Answered: An Hfr strain that is lacZ and galE"…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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If the intact chromosomes of E. coli are labeled with heavy nitrogen (15N), then E. coli is fed light nitrogen (14N), what would be the expected distribution of DNA by the fourth (F4) generation? 12.5% intermediate-weight DNA, and 87.5% light-weight DNA 25% intermediate-weight DNA, and 75% heavy-weight DNA 50% light-weight DNA, and 50% intermediate-weight DNA 75% intermediate-weight DNA, and 25% heavy-weight DNA 87.5% intermediate-weight DNA, and 12.5% heavy-weight DNA The above experiment, on DNA replication in the intact chromosomes of E. coli (with no virus infection) was first carried out by which of the following investigators? Rosalind Franklin and Maurice Wilkins Reiji Okazaki and Tuneko Okazaki Matthew Meselson and Franklin Stahl James Watson and Francis Crick Erwin Chargaff and Arthur Kornberg
You've crossed two strains: (Hfr) pro+ lac+ arg+ with (F-) pro- lac- arg- . Interrupted mating studies indicated that arg+ enters the cell last, so you want to select for arg+ recombinants in order to map the genes by recombination. Which type of plates will you use for your initial selection? Containing proline, lactose, and arginine. Containing proline, glucose, and arginine. Containing arginine and glucose. Containing lactose and proline. Containing glucose and proline. Minimal media with nothing added.
An interrupted mating study is carried out on Hfr strains 1, 2, and 3. After conjugation is established, a small sample of the mixture is collected every minute for 20 minutes to determine the distance between the genes on the chromosome. Times of entry for each of the three Hfr strains studied in the interrupted mating are provided in parenthesis. The total duration of conjugation (in minutes) is given for each transferred gene. Hfr Strain Genes Transferred Hfr 1 oriT(0) met(2) ala(8) lac(13) gal(17) Hfr 2 oriT(0) met(2) leu(7) thr(10) azi(17) Hfr 3 oriT (0) gal(3) pro(8) trp(14) azi(19) Hfr 4 oriT leu met ala lac Hfr 5 oriT trp azi thr leu a. Use the data to construct a circular map of the bacterium.
Compared to the normal A allele, the disease-causing allele in sickle cell anemia (S allele) is missing an MstII restriction site. On a Southern blot of genomic DNA cut with MstII and hybridized with the probe shown on the diagram below, a person with sickle anemia, carrying two S alleles, will show Choose an answer below: a single band at 1.1 kb. a single band at 1.3 kb. a single band at 0.2 kb. one band at 0.2 and one at 1.3 kb. one band at 1.1 and one at 1.3 kb.
The haploid human genome consists of approximately 3.2 × 109 bp (or 6.4 × 109 bp per diploid genome). Assuming that DNA is B-DNA, calculate the total length of a single cell’s DNA.
Please find the attachment
A particular Hfr strain normally transmits the pro+marker as the last one in conjugation. In a cross of thisstrain with an F− strain, some pro+ recombinants are recovered early in the mating process. When these pro+cells are mixed with F−cells, the majority of the F− cellsare converted into pro+ cells that also carry the F factor.Explain these results.
Instructions: — Please answer properly — Donot copy here in Bartleby or in Google.
Below are two pictures of perithecium on microscope slides that have been squished under cover slips so that the asci inside have spread out. In the asci, we can clearly see the tan ascospores (light colored) and wildtype ascospores (dark colored). We will perform our tetrad analysis by counting how many of the asci are non-recombinant and how many of the acsi are recombinant. We can tell which are recombinant and non-recombinant by observing the patterns of the ascospores in the asci. See the patterns of ascospores for non- recombinant and recombinant asci in the table below. Ascospore pattern of non-recombinant asci OOOO OOOOO You will count the total number of non-recombinant and recombinant asci in the two pictures below. You will only count the asci that have a star next to them because these asci can be clearly identified as non-recombinant or recombinant. Select the correct number below after you are done. Total number of non-recombinant asci [Select] Ascospore pattern of…
If you mated an F' bacterial cell with lacl+ lacP+ lacO+ lacZ+ lacY+ on the bacterial chromosome and lacl+ lacP+ lacOc lacZ+ lacY- on a plasmid with an F- lacl+ lacP+ lacO+ lacZ+ lacY+ and lactose was absent and glucose was absent, what expression levels do you predict in the RECIPENT cell after conjugation (assume no recombination)? Betagalactosidase expression will be HIGH Betagalactosidase expression will be BASAL Betagalactosidase expression will be LOWER THAN BASAL There will be NO Betagalactosidase expression
An Hfr strain that is leuA+ and thiL+ was mixed with a strain thatis leuA− and thiL−. In the data points shown in the following graph,the conjugation was interrupted at different time points, and thepercentage of recombinants for each gene was determined bystreaking on a medium that lacked either leucine or thiamine.Make a calculation.

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