Percentage of cells transformed into Transforming DNA str" mtl- str* mtl+ str" mtl+ str" mtl+ str" mtl- + str® mtl+ 4.3 0.40 0.17 2.8 0.85 0.0066

Q: T. Miyake and M. Demerec examined proline-requiring mutations in the bacterium Salmonella…

A: In transduction process, the closer the genes are present on a DNA, the more possibility they have…

Q: BACTERIA STRAIN A IS AUXOTROPHIC FOR METHIONINE AND STRAIN B IS AUXOTROPHIC FOR LEUCINE. A. WILL…

A: Auxotrophic strains lack the property of synthesising the growth factors required for their proper…

Q: Bacteriophage P22 was used in generalized transduction experiments to infect the Salmonella…

A: The horizontal transfer of the genetic material from donor bacteria to the recipient bacteria via…

Q: It was shown that the pan marker entered last in interrupted conjugation experiments. By spreading…

A: Minimal media is the media which required minimum component for the growth.  Here MM+ glucose = 280…

Q: When you go to the lab and you carry out the reaction several times, you realize that you made the…

A: The polymerase chain reaction is a technique in molecular biology that is used to amplify the target…

Q: Describe what each of the 12 bands on your Western blot should have been. Remember, the 12 bands…

A: Western blotting: It is a technique used to identify and locate proteins based on their ability to…

Q: In the Avery, McCarty and McCleod experiment, DNA was identified as the heritable material because,…

A: A long time back it was unknown which the genetic material among protein, RNA, and DNA. It was later…

Q: Following a dye terminator DNA sequencing reaction using 2',3'-dideoxynucleotide triphosphates…

A: Dye terminator DNA sequencing is the modern version of Sanger sequencing. It uses…

Q: A second enzyme has 4 differer fragments. If both enzymes are you took all the fragments from…

A: Restriction enzyme is a protein produced by bacteria that cleaves DNA at specific sites along the…

Q: Explain

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Q: In a cotransduction experiment involving P1, the cotransductionfrequency was 0.53. How far apart are…

A: Transduction is the process of the transferring foreign DNA into the host cell by viral vector. T4…

Q: An Hfr strain of E. coli that is pro thr * leu * lac " was "mated" with an F- strain that is pro thr…

A: Introduction Genetic recombination is a process of exchange of genetic material between two…

Q: Can you answer the last three q

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Q: I know the answer is e but can you thoroughly explain the answer choices? like what does…

A: The prokaryotic gene regulation is known as operon system which regulates the transcription of a…

Q: In the experiment below, flasks of human fibroblasts are either transfected with a control gene or…

A: The cell division is the process by which a single mother cell divides and produce two or more…

Q: DNA obtained from the indicated donor type recombinants are scored. The number of wild-types for…

A: Recipient lacking in b and c genes (a+ b-c-) has the largest number of transformed colonies(462

Q: A recombinant protein corresponding to 52 repeats of the peptide sequence YSPTSPS was covalently…

A: The peptide sequence YSPTSPS is a known phosphorylation site that corresponds to the heptad repeat…

Q: An Hfr strain that is hisE + and pheA + was conjugated to a strain that is hisE − and pheA −. The…

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Q: In an experiment assay, sometimes there are several dilutions involved. 0.5 mL of (Bovine serum…

A: Dilution is responsible for decreasing the solute concentration in the solution.

Q: Bacteriophage P22 was used in generalised transduction experiments to infect the Salmonella…

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Q: Compatible ends generated by restriction enzyme can be connected by ligase. Determine which of the…

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A: The genetic alterations of a cell that results from the direct uptake and integration of exogenous…

Q: An Hfr strain that is hisE+ and pheA+ was mixed with a strain thatis hisE− and pheA−. The…

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Q: Can you please help with 1b please. picture with 1 graph is for question 1a) picture with 4 graphs…

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Q: A fluctuation test was carried out to determine the rate of mutation to Azetidine resistance (a…

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Q: DNA containing the genes gin+ phe+ lys+ was used to transform bacterial cells of genotype gln- phe-…

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Q: DNA obtained from the indicated donor strains is used to TRANSFORM the indicated recipient strains.…

A: The correct answer is option C. Arginine The arginine locus is closest to serine.

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Q: Four E. coli strains of genotype a+ b- are labeled 1, 2, 3, and 4. Four strains of genotype a- b+…

A: A gene is the essential physical and functional unit of heredity. They are comprised of DNA…

Q: Choose two genes from Figure 4.6 and draw a graph to represent the change in transcription over…

A: Transcription:  All the necessary information required to make proteins is stored n the nucleotide…

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Q: DNA from a strain of Bacillus subtilis with genotype a+ b+ c+ d+ e+ is used to transform a strain…

A: Co transformation means that the two genes are transformed simultaneously. Is two genes are co…

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Q: Mutants tested 1,2 1,3 1,4 1,5 Which would be a possible arrangement of mutations on the two rll…

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Q: what does it mean if my P value is P< .001 for average absorbance ratios of A260/A280 of dna…

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Q: strain of E. coli. Timed matings showed that they transferred the following loci during the first…

A: Bacteria are prokaryotic, unicellular organism. Bacteria belong to the kingdom Monera. Bacteria…

Q: Cystic fibrosis (CF) is a genetic disorder affecting a number of organs, including the lung airways,…

A: many types of mutations are present which can explain the cause of the data we see above as cystic…

Q: following recombinants recover when conjugation occurs between an a+d+g+ donor and an a d'g…

A: A gene control all the characters in living organisms. A gene is the structural and functional unit…

Q: A prototrophic strain (his" arg" lac') was used as a donor to transform an auxotrophic strain (his…

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Q: A 2.0kb bacterial plasmid ‘BS1030’ is digested with the restriction endonuclease Sau3A; the plasmid…

A: Plasmids are small, circular, double stranded, and extrachromosomal bacterial DNA. i.e. it is…

Q: Using a named example, discuss the advantages of using E. coli to produce recombinant proteins.

A: Recombinant DNA technology defines the artificial manipualtion of the genetic material to create the…

Q: Compared to the normal A allele, the disease-causing allele in sickle cell anemia (S allele) is…

A: Restriction enzymes are molecular scissors.

Q: The probe in our telomere experiment was labeled with digoxigenin. What are the advantages of using…

A: Digoxigenin is a small molecule that can be covalently attached to DNA or RNA probes to label them…

Q: T. Miyake and M. Demerec examined proline-requiring mutations in the bacterium Salmonella…

A: Transduction is the process by which a virus transfers genetic material from one bacterium to…

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.