As the leading scientist in a biomedical science laboratory, it is a requirement to give advice to your lab assistants when they are having problems with their experiments. What advice would you give to your assistants that are having the following problems: After performing a polymerase chain reaction (PCR) and agarose gel electrophoresis to confirm the presence of the CO1 gene of 750bp.

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
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As the leading scientist in a biomedical science laboratory, it is a requirement to give advice to your lab
assistants when they are having problems with their experiments. What advice would you give to your
assistants that are having the following problems: After performing a polymerase chain reaction (PCR)
and agarose gel electrophoresis to confirm the presence of the C01 gene of 750bp.
2.1. They observe no band appearing on an agarose gel. What would be your conclusion?
2.2. They observe three bands of different sizes that resemble a smear on the gel. Advice
2.3. They observe a single band on the gel and conclude that the PCR product is an exact copy of
the original template DNA. Would you support their condusion? Explain.
2.4. Explain how PCR can be used to detect infectious agents in diagnoses of diseases.
Transcribed Image Text:As the leading scientist in a biomedical science laboratory, it is a requirement to give advice to your lab assistants when they are having problems with their experiments. What advice would you give to your assistants that are having the following problems: After performing a polymerase chain reaction (PCR) and agarose gel electrophoresis to confirm the presence of the C01 gene of 750bp. 2.1. They observe no band appearing on an agarose gel. What would be your conclusion? 2.2. They observe three bands of different sizes that resemble a smear on the gel. Advice 2.3. They observe a single band on the gel and conclude that the PCR product is an exact copy of the original template DNA. Would you support their condusion? Explain. 2.4. Explain how PCR can be used to detect infectious agents in diagnoses of diseases.
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