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Component 1 PCR buffer 2 MgCl2 3 dNTP mix 4 SSR forward primer 5 SSR reverse primer 6 Taq polymerase 7 Sterile nanopure water Initial Concentration 10x 25 mM 10 mM 5 U/μL Final Concentration 1x 1.25 mM 0.4 mM 0.1 μM 0.1 μM 0.5 U/reaction Volume (μL) for 1x 1.5 0.6 0.3 0.3 9.45 Volume (μL) for X 7.5

Component 1 PCR buffer 2 MgCl2 3 dNTP mix 4 SSR forward primer 5 SSR reverse primer 6 Taq polymerase 7 Sterile nanopure water Initial Concentration 10x 25 mM 10 mM 5 U/μL Final Concentration 1x 1.25 mM 0.4 mM 0.1 μM 0.1 μM 0.5 U/reaction Volume (μL) for 1x 1.5 0.6 0.3 0.3 9.45 Volume (μL) for X 7.5

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Run a barcoding protocol with rbcL gene-specific primers. Use 30 cycles for your PCR amplification profile. Complete the table below for your PCR cocktail.

Initial Final Volume (ul) Volume (ul) Component Concentration Concentration for 1x for 1 PCR buffer 10x 1x 1.5 7.5 2 MgCl2 25 mM 1.25 mM 3 DNTP mix 10 mM 0.4 mM 0.6 4 SSR forward primer 0.1 μΜ 0.3 5 SSR reverse primer 0.1 μΜ 0.3 6 Taq polymerase 5 U/ul 0.5 U/reaction 7 Sterile nanopure water 9.45 --- 8 8DNA ng/ul 150 ng/reaction 2 .-- Total Volume 15
Transcribed Image Text:Initial Final Volume (ul) Volume (ul) Component Concentration Concentration for 1x for 1 PCR buffer 10x 1x 1.5 7.5 2 MgCl2 25 mM 1.25 mM 3 DNTP mix 10 mM 0.4 mM 0.6 4 SSR forward primer 0.1 μΜ 0.3 5 SSR reverse primer 0.1 μΜ 0.3 6 Taq polymerase 5 U/ul 0.5 U/reaction 7 Sterile nanopure water 9.45 --- 8 8DNA ng/ul 150 ng/reaction 2 .-- Total Volume 15
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