Genetic Recombination
Recombination is crucial to this process because it allows genes to be reassorted into diverse combinations. Genetic recombination is the process of combining genetic components from two different origins into a single unit. In prokaryotes, genetic recombination takes place by the unilateral transfer of deoxyribonucleic acid. It includes transduction, transformation, and conjugation. The genetic exchange occurring between homologous deoxyribonucleic acid sequences (DNA) from two different sources is termed general recombination. For this to happen, an identical sequence of the two recombining molecules is required. The process of genetic exchange which occurs in eukaryotes during sexual reproduction such as meiosis is an example of this type of genetic recombination.
Microbial Genetics
Genes are the functional units of heredity. They transfer characteristic information from parents to the offspring.
Four E. coli strains of genotype a+ b- are labeled 1, 2, 3,
and 4. Four strains of genotype a- b+ are labeled 5, 6, 7,
and 8. The two genotypes are mixed in all possible combinations and (after incubation) are plated to determine the
frequency of a+ b+ recombinants. The following results
are obtained, where M = many recombinants, L = low
numbers of recombinants, and 0 = no recombinants:On the basis of these results, assign a sex type (either
Hfr, F+, or F-) to each strain.
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