When you go to the lab and you carry out the reaction several times, you realize that you made the following mistakes in your different trials. What would it be the outcome in each case? Justify your answers. 1. You forgot to add one primer 2. You forgot to add dGTP 3. You added primers in the wrong concentration, and they are not in excess compared to the dsDNA. 4. You add all the required components but you heat to 95°C only once.

When you go to the lab and you carry out the reaction several times, you realize that you made the following mistakes in your different trials. What would it be the outcome in each case? Justify your answers. 1. You forgot to add one primer 2. You forgot to add dGTP 3. You added primers in the wrong concentration, and they are not in excess compared to the dsDNA. 4. You add all the required components but you heat to 95°C only once.

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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