Recombinant protein production by a genetically modified Escherichia coli strain is proportional to cell growth. Ammonia is used as a nitrogen source for aerobic glucose respiration. The recombinant protein has the general formula CH1,55O0,31N0,25, while that of the cellular biomass is CH1,77O0,49N0,24. The biomass yield from glucose equals 0.50 g/g, while the recombinant protein yield from glucose corresponds to 20% of the cell yield from substrate. a) How much ammonia is required? What is the oxygen demand? (b) If the biomass yield remains the same, what are the ammonia and oxygen requirements for a wild-type strain of E. coli, with cell biomass of the same elemental composition, but unable to synthesize the recombinant protein? (c) On an industrial scale, cultivation takes place in a continuous fermenter at 28°C and the desired recombinant protein production rate is 7 g/h. Since the viscosity of the culture broth is considerable, the energy input due to agitation cannot be neglected. The fermenter is equipped with a turbine which, operating at a power of 15 kW, provides good mixing and aeration. Estimate how much energy must be removed from the system to keep the temperature at 28°C.
Recombinant protein production by a genetically modified Escherichia coli strain is proportional to cell growth. Ammonia is used as a nitrogen source for aerobic glucose respiration. The recombinant protein has the general formula CH1,55O0,31N0,25, while that of the cellular biomass is CH1,77O0,49N0,24. The biomass yield from glucose equals 0.50 g/g, while the recombinant protein yield from glucose corresponds to 20% of the cell yield from substrate.
a) How much ammonia is required? What is the oxygen demand?
(b) If the biomass yield remains the same, what are the ammonia and oxygen requirements for a wild-type strain of E. coli, with cell biomass of the same elemental composition, but unable to synthesize the recombinant protein?
(c) On an industrial scale, cultivation takes place in a continuous fermenter at 28°C and the desired recombinant protein production rate is 7 g/h. Since the viscosity of the culture broth is considerable, the energy input due to agitation cannot be neglected. The fermenter is equipped with a turbine which, operating at a power of 15 kW, provides good mixing and aeration. Estimate how much energy must be removed from the system to keep the temperature at 28°C.
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