Assignment_12.pdf - Adobe ReaderFile Edit View Window HelpОpen1/ 199,1%Tools Fill & Sign Comment3. Recombinant protein is produced by a genetically engineered strain of Escherichia coli during cellgrowth. The recombinant protein can be considered a product of cell culture even though it is notsecreted from the cells; it is synthesized in addition to normal E. coli biomass. Ammonia is used asthe nitrogen source for aerobic respiration of glucose.The recombinant protein has an overall formula of CH1.5500.31N..25. The yield of biomass (excludingrecombinant protein) from glucose is measured as o.48 g/g; the yield of recombinant protein fromglucose is about 20% of that for cells.How much ammonia is required?What is the oxygen demand?If the biomass yield remains at 0.48 g /g, how much different are the ammonia and oxygenrequirements for wild-type E. coli that is unable to synthesize recombinant protein?а.b.с.24°C08:39Berawan27/04/2022

Disulfide-bonded proteins primarily mature in the oxidative environment of the eukaryotic…

Answered: 3. Recombinant protein is produced by a…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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In E. coli, Base excision repair (BER) handles non-bulky lesions (e.g., deamination, oxidation) and nucleotide excision repair (NER) handles bulky lesions (e.g., Py dimers). Is this statement true or false? O True False
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You are given two samples of DNA, one from Clostridium perfringens and the other from Mycobacterium tuberculosis. Unfortunately, the labels on the tubes of DNA were accidentally removed, and you do not know which DNA sample belongs to which organism. As you were doing some work with Escherichia coli in the lab, you also have some of its DNA available as well. The %G + %C values for each of the organisms is known (C. perfringens, 27%; M. tuberculosis, 67%; E. coli, 50%). Based upon this information, how could you quickly determine the source of the DNA for your two samples?
1) Provide a stepwise, arrow-pushing mechanism for the following transformation. You may use general acid and base as necessary. Feel free to abbreviate structures as needed. مسلسلند SCOA Lyase TPP mos مسلسلند H + H 요 SCOA
Under what conditions will the following partial diploid strain of E. coli convert chorismate to tryptophan (assume trp mutants function similarly to lac mutants)? trpR* trpP trpO* trpE* trpD* trpC* trpB* trpA*/ trpR trpP trpO° trpE trpD trpC trpB trpA ect the one BEST answer (2 oints! O never O only when tryptophan levels are low O constitutively O only when tryptophan is present O only when chorismate levels are low
Please answer the following questions using numbers and not text. For example, if you think the answer is 24, write 24 and not twenty-four. If you think the answer is 24 ATP, write only the number 24. As a microbiology technician, your job is to characterize a novel bacterial strain isolated from insects. Phenotypically, you have discovered that the new bacterial strain can grow in the presence or absence of oxygen. After genome sequencing and biochemical characterization, you know that your new organism is capable of making all of the enzymes necessary for glycolysis and the TCA cycle, and that it has an electron transport chain. Answer the following questions based on the growth of your organism on minimal media plates containing only acetate as a carbon and energy source. Acetate is transported across the membrane at the cost of 3 ATP per molecule. Each question should be answered based on the metabolism of one molecule of acetate. If you come up with a negative value, indicate an…
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what are the genotypes of the following e.coli strains 1-4?

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