Assignment_12.pdf - Adobe ReaderFile Edit View Window HelpОpen1/ 199,1%Tools Fill & Sign Comment3. Recombinant protein is produced by a genetically engineered strain of Escherichia coli during cellgrowth. The recombinant protein can be considered a product of cell culture even though it is notsecreted from the cells; it is synthesized in addition to normal E. coli biomass. Ammonia is used asthe nitrogen source for aerobic respiration of glucose.The recombinant protein has an overall formula of CH1.5500.31N..25. The yield of biomass (excludingrecombinant protein) from glucose is measured as o.48 g/g; the yield of recombinant protein fromglucose is about 20% of that for cells.How much ammonia is required?What is the oxygen demand?If the biomass yield remains at 0.48 g /g, how much different are the ammonia and oxygenrequirements for wild-type E. coli that is unable to synthesize recombinant protein?а.b.с.24°C08:39Berawan27/04/2022

Disulfide-bonded proteins primarily mature in the oxidative environment of the eukaryotic…

Answered: 3. Recombinant protein is produced by a…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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In E. coli, Base excision repair (BER) handles non-bulky lesions (e.g., deamination, oxidation) and nucleotide excision repair (NER) handles bulky lesions (e.g., Py dimers). Is this statement true or false? O True False
Three pairs of bacterial cells with the given genotypes undergo conjugation. Place match the genotype of each cell after conjugation to its initial genotype. F+ x F Hfr F- F' F- Answer Bank F F F+ Hfr What is the role of the F-factor in conjugation? It contains genes necessary for replication of the donor's F plasmid. can occur. It allows auxotrophic bacterial cells to survive on minimal medium so that conjugation It contains genes that force recombination between the donor and recipient chromosomes. It contains genes necessary for the formation of the pilus. O It degrades the chromosome of the recipient cell after conjugation.
you have transformed E.coli cells with a plasmid containing the gene for the enzyme beta-lactamase you have used 3µl of a stock solution of 2.5ng/µl DNA. You transformed 150/µl of competent cells, using the calcium chloride method. you have added 600µl of luria broth to the cells and then plated out 200µl on triplicate plates. upon counting the colonies, you got the following results: plate 1: 120 cfu plate 2: 35 cfu plate 3: 95 cfu please answer the following question: 1. what new characteristic will the cells have ater transformation? 2. how are you going to test for the new characteristic? 3. calculate the transformation efficiency of the experiment?
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Choices are in the picture. Choose the primary impact of the given agents to the genetic material. 6. Oxidative free radicals 7. Methotrexate 8. Illegitimate recombination 9. X-rays 10. Daunorubicin
1) Provide a stepwise, arrow-pushing mechanism for the following transformation. You may use general acid and base as necessary. Feel free to abbreviate structures as needed. مسلسلند SCOA Lyase TPP mos مسلسلند H + H 요 SCOA
Under what conditions will the following partial diploid strain of E. coli convert chorismate to tryptophan (assume trp mutants function similarly to lac mutants)? trpR* trpP trpO* trpE* trpD* trpC* trpB* trpA*/ trpR trpP trpO° trpE trpD trpC trpB trpA ect the one BEST answer (2 oints! O never O only when tryptophan levels are low O constitutively O only when tryptophan is present O only when chorismate levels are low
Please answer the following questions using numbers and not text. For example, if you think the answer is 24, write 24 and not twenty-four. If you think the answer is 24 ATP, write only the number 24. As a microbiology technician, your job is to characterize a novel bacterial strain isolated from insects. Phenotypically, you have discovered that the new bacterial strain can grow in the presence or absence of oxygen. After genome sequencing and biochemical characterization, you know that your new organism is capable of making all of the enzymes necessary for glycolysis and the TCA cycle, and that it has an electron transport chain. Answer the following questions based on the growth of your organism on minimal media plates containing only acetate as a carbon and energy source. Acetate is transported across the membrane at the cost of 3 ATP per molecule. Each question should be answered based on the metabolism of one molecule of acetate. If you come up with a negative value, indicate an…
You are studying a type of bacteria isolated from the acidic water runoff of a mining operation. You subject two batches of the same bacteria type to different environmental growth conditions. One batch is grown at pH 2, while the other is grown at pH 7. All other environmental parameters are kept identical between the two batches. You then collect their proteins and run a Western blot using an antibody that binds to a proton efflux pump protein (which actively expends energy to pump protons out of a cell). How would you characterize the information obtained in this experiment? What does it tell you, and why is that potentially valuable information?
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