monomer and MamP dimer (disulfide bonded). Why does the MamP monomer run faster on the gel relative to the MamP dimer?

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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1. In the SDS-PAGE figure below, looking at lane 6 there are two bands MamP,
monomer and MamP dimer (disulfide bonded).
Why does the MamP monomer run faster on the gel relative to the MamP dimer?
1 2 3 4 5 6
250 -
130 -
100
70
55
- Hisg MBPtev.Mamf
MamP dimer
35 -
25-
- MamP monomer
15 -
10 -
If the gel was run under non-reducing conditions, would the pattern possibly be
different and why?
Molecular weight (kD)
Transcribed Image Text:1. In the SDS-PAGE figure below, looking at lane 6 there are two bands MamP, monomer and MamP dimer (disulfide bonded). Why does the MamP monomer run faster on the gel relative to the MamP dimer? 1 2 3 4 5 6 250 - 130 - 100 70 55 - Hisg MBPtev.Mamf MamP dimer 35 - 25- - MamP monomer 15 - 10 - If the gel was run under non-reducing conditions, would the pattern possibly be different and why? Molecular weight (kD)
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