The image below is an example of the results that you could expect to see from your gel. Each group (e.g., lanes labelled A) contains two samples, Bl=Blue and Br=Brown. Interpret the results below as being homozygous blue, homozygous brown, or heterozygous. A В LMW C BI Br BI Br BI Br BI Br A. В. 250 С. 200 150 D. The sample lane listed as "LMW" denotes what is known as a Low Molecular Weight marker See Figure3This is a commercially available sample set of standard DNA fragments, measured in base pairs (bp). These standards of known size are used to determine the size of your PCR products (the DNA fragments you have created by PCR) by comparing the position of your bands with the bands of known size. Based on the gels above and the image of the LMW marker below, extrapolate the size of the Blue and Brown eye colour alleles. 766 Blue eye colour allele size (bp): 500 350 Brown eye colour allele size (bp): 300 250 200 Figure 3: Low Molecular Weight DNA Marker (New England Biolabs) 150 100 75 -50 25
The image below is an example of the results that you could expect to see from your gel. Each group (e.g., lanes labelled A) contains two samples, Bl=Blue and Br=Brown. Interpret the results below as being homozygous blue, homozygous brown, or heterozygous. A В LMW C BI Br BI Br BI Br BI Br A. В. 250 С. 200 150 D. The sample lane listed as "LMW" denotes what is known as a Low Molecular Weight marker See Figure3This is a commercially available sample set of standard DNA fragments, measured in base pairs (bp). These standards of known size are used to determine the size of your PCR products (the DNA fragments you have created by PCR) by comparing the position of your bands with the bands of known size. Based on the gels above and the image of the LMW marker below, extrapolate the size of the Blue and Brown eye colour alleles. 766 Blue eye colour allele size (bp): 500 350 Brown eye colour allele size (bp): 300 250 200 Figure 3: Low Molecular Weight DNA Marker (New England Biolabs) 150 100 75 -50 25
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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