A cross is made between two E. coli strains: Hfr arg + bio + leu+ × F− arg − bio − leu−. Interrupted mating studies show that arg+ enters the recipient last, and so arg+ recombinants are selected on a medium containing bio and leu only. These recombinants are tested for the presence of bio + and leu+. The following numbers of individuals are found for each genotype: arg+ bio+ leu+ 320 arg+ bio- leu+ 0 arg+ bio+ leu- 8 arg+ bio- leu- 48a. What is the gene order? b. What are the map distances in recombination percentages?
Genetic Recombination
Recombination is crucial to this process because it allows genes to be reassorted into diverse combinations. Genetic recombination is the process of combining genetic components from two different origins into a single unit. In prokaryotes, genetic recombination takes place by the unilateral transfer of deoxyribonucleic acid. It includes transduction, transformation, and conjugation. The genetic exchange occurring between homologous deoxyribonucleic acid sequences (DNA) from two different sources is termed general recombination. For this to happen, an identical sequence of the two recombining molecules is required. The process of genetic exchange which occurs in eukaryotes during sexual reproduction such as meiosis is an example of this type of genetic recombination.
Microbial Genetics
Genes are the functional units of heredity. They transfer characteristic information from parents to the offspring.
A cross is made between two E. coli strains: Hfr arg
+ bio
+
leu+ × F− arg
− bio
− leu−. Interrupted mating studies
show that arg+ enters the recipient last, and so arg+ recombinants are selected on a medium containing bio and
leu only. These recombinants are tested for the presence
of bio
+ and leu+. The following numbers of individuals
are found for each genotype:
arg+ bio+ leu+ 320 arg+ bio- leu+ 0
arg+ bio+ leu- 8 arg+ bio- leu- 48a. What is the gene order?
b. What are the map distances in recombination
percentages?
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