. a. You want to perform an interrupted-mating mappingwith an E. coli Hfr strain that is Pyr+, Met+, Xyl+,Tyr+, Arg+, His+, Mal+, and Strs. Describe anappropriate bacterial strain to be used as theother partner in this mating.b. In an Hfr × F− cross, the pyrE gene enters therecipient in 5 minutes, but at this time point thereare no exconjugants that are Met+, Xyl+, Tyr+,Arg+, His+, or Mal+. The mating is now allowed toproceed for 30 minutes and Pyr+ exconjugants areselected. Of the Pyr+ cells, 32% are Met+, 94% areXyl+, 7% are Tyr+, 59% are Arg+, 0% are His+, and71% are Mal+. What can you conclude about theorder of the genes?
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
. a. You want to perform an interrupted-mating mapping
with an E. coli Hfr strain that is Pyr+, Met+, Xyl+,
Tyr+, Arg+, His+, Mal+, and Strs
. Describe an
appropriate bacterial strain to be used as the
other partner in this mating.
b. In an Hfr × F− cross, the pyrE gene enters the
recipient in 5 minutes, but at this time point there
are no exconjugants that are Met+, Xyl+, Tyr+,
Arg+, His+, or Mal+. The mating is now allowed to
proceed for 30 minutes and Pyr+ exconjugants are
selected. Of the Pyr+ cells, 32% are Met+, 94% are
Xyl+, 7% are Tyr+, 59% are Arg+, 0% are His+, and
71% are Mal+. What can you conclude about the
order of the genes?
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
