After much work the researcher maps the mutation in the mut1 plant to a locus on chromosome 2.  The gene associated with the mut1 mutation is called COB1 (i.e. mutations in COB1 cause the mut1 phenotype.) The researcher extracts DNA and RNA from developing ears of wild type, mut1 and mut2 plants.  From the DNA samples COB1 PCR primers are used to amplify the coding region of the COB1 gene (see DNA gel below).  An RNA gel is used to examine the expression of COB1 all three plants (see RNA gel below).  results in photo: Which of the following is TRUE about the COB1 gene.  a. There is a deletion in the COB1 gene in the mut1 plants. b. COB1 is not transcribed in the mut2 plant. c. There is a deletion in the COB1 gene in the mut2 plants. d. COB1 is not transcribed in the mut1 plant.

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
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After much work the researcher maps the mutation in the mut1 plant to a locus on chromosome 2.  The gene associated with the mut1 mutation is called COB1 (i.e. mutations in COB1 cause the mut1 phenotype.)

The researcher extracts DNA and RNA from developing ears of wild type, mut1 and mut2 plants.  From the DNA samples COB1 PCR primers are used to amplify the coding region of the COB1 gene (see DNA gel below).  An RNA gel is used to examine the expression of COB1 all three plants (see RNA gel below).  results in photo:

Which of the following is TRUE about the COB1 gene. 

a. There is a deletion in the COB1 gene in the mut1 plants.
b. COB1 is not transcribed in the mut2 plant.
c. There is a deletion in the COB1 gene in the mut2 plants.
d. COB1 is not transcribed in the mut1 plant.
WT
mut1
mut2
DNA gel showing
COB1 gene
RNA gel showing
COB1 MRNA
Transcribed Image Text:WT mut1 mut2 DNA gel showing COB1 gene RNA gel showing COB1 MRNA
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