A research group discovers a new version of happyase, which they call happyase*, that catalyzes the chemical reaction: HAPPY.-SAD The researchers begin to characterize the enzyme. The researchers determined the kcat of the enzyme to be 395 s1 and the Vmax to be 1.6 µM s1. In a separate experiement with (E) at 1.5 nM and (HAPPY) at 35 pM, the researchers find that Vo is equal to 250 nM s-1. what is the measured Km of happyase* for its substrate HAPPY? Make sure to include units in the answer.

Biochemistry
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ISBN:9781319114671
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Chapter1: Biochemistry: An Evolving Science
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A research group discovers a new version of happyase, which they call happyase*, that catalyzes the chemical reaction:
HAPPY--SAD
The researchers begin to characterize the enzyme. The researchers determined the kcat of the enzyme to be 395 s1 and the
Vmax to be 1.6 µM s1. In a separate experiement with [E) at 1.5 nM and [HAPPY] at 35 µM, the researchers find that Vo is equal
to 250 nM s-1. What is the measured Km of happyase* for its substrate HAPPY? Make sure to include units in the answer.
Transcribed Image Text:A research group discovers a new version of happyase, which they call happyase*, that catalyzes the chemical reaction: HAPPY--SAD The researchers begin to characterize the enzyme. The researchers determined the kcat of the enzyme to be 395 s1 and the Vmax to be 1.6 µM s1. In a separate experiement with [E) at 1.5 nM and [HAPPY] at 35 µM, the researchers find that Vo is equal to 250 nM s-1. What is the measured Km of happyase* for its substrate HAPPY? Make sure to include units in the answer.
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