A purified recombinant protein is analyzed for molecular weight by SDS-PAGE at pH 8.5. From the protein sequence deduced from the gene that was expressed in bacteria, the protein is expected to have a molecular weight of 44,000. However, the molecular weight of the protein is

Q: You observe the following: Your Your friend's Gel Gel 120 kDa 100 kDa 120 kDa 100 kDa -80 kDa 80 kDa…

A: you have asked multiple question please repost the question and mention the sub parts needed to be…

Q: Describe the banding pattern seen in the gel. What does that tell you about the role of annealing…

A: Gel electrophoresis is a laboratory technique for separating charged molecules such as DNA, RNA, and…

Q: A thermophillic bacterium was isolated from a hot spring at Perak to study its thermostable amylase…

A: DNA technology is the chemical manipulation of the genotype and the resulting phenotype of organisms…

Q: Are all the proteins separated properly using SDS-PAGE? Why or why not?

A: Proteins are massive biomolecules and macromolecules made up of one or more long chains of amino…

Q: 4.08 2.54 H Note: E E 1.02 1.02 1.67 H = EXON = INTRON AE E 10.8 kbp 3.94 3.66 E = EcoRI site H =…

A: Autoradiography is a method that visualizes molecules or fragments of molecules that have been…

Q: starting and ending purity, t

A: Purification of a Recombinant Protein:This process involves the following steps-Cel harvesting- In…

Q: A very common molecular biology research method is to analyze cell or tissue homogenates by…

A: In molecular biology research, SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting,…

Q: 1)What is the objective of hydrolyzing the RNA prior to doing biochemical tests? 2) What particular…

A: Asked : Objective of RNA hydrolysis  Test for pentose except Molisch's  RNA and DNA questions are…

Q: You have purified a recombinant form of the p53 protein from E. coli and determined the A280 to be…

A: Beers law states that the absorbance of a sample depends on the amount of sample present and path…

Q: Analysis of a 49kDA protein is aimed with a western blot technique. For this purpose, "whole cell…

A: Western blot is a technique in which the concentration of a protein is determined using SDS PAGE…

Q: please solve this step by step with clear explaination

A: To convert the given amounts of DNA from micrograms (μg) to microliters (μl), we need to use the…

Q: The following list of proteins are found in a mixture and are to be separated by 2D gel…

A: In 2D gel electrophoresis, is a 2 step-based separation of protein.  In the first dimension, the…

Q: A recombinant protein corresponding to 52 repeats of the peptide sequence YSPTSPS was covalently…

A: The peptide sequence YSPTSPS is a known phosphorylation site that corresponds to the heptad repeat…

Q: Scenario 1: DNA concentration? UV-Vis spectroscopy [Choose ] Homology Modeling Scenario 2: Does it…

A: Different techniques are used for determining different properties.

Q: a)Dr. Thisisaneasyexam decides to amplify a gene from a plasmid using PCR. She starts out with 6.6 x…

A: Sure, let me provide a more detailed explanation for the calculations.a) Calculating the number of…

Q: Shown below is an R loop prepared for electron microscopy by annealing a purified eukaryotic…

A: An exon is any part of a gene that will encode a part of the final mature RNA produced by that gene…

Q: If your analysis identifies that the total Cytosine content in a sample is 29%, what will be the…

A: According to Chargaff's rule number of purine(adenine+guanine) = number of pyrimidine (cytosine +…

Q: The practical limit for the number of different RNA sequences screened in a SELEX experiment is…

A: SELEX stands for Systematic Evolution of Ligands by EXponential enrichment. It is a laboratory…

Q: Expressing genes encoding a particular protein’s subunits from a plasmid in E. coli cells (volume of…

A: Escherichia coli (E. coli) are group of bacteria that are prevalent in the environment, foods, and…

Q: You will have to dilute your inital Lysozyme stock in order to pipet volumes larger than 10 uL for…

A: Step 1:For the Bradford assay, the typical concentration range of protein you want to target is…

Q: pMDawn is digested with EcoR1, and BamHI. Resulting in fragments shown below: EcoRI: 20 kb BamHI:…

A: Agarose gel electrophoresis is a method of gel electrophoresis that is used to separate a mixed…

Q: SDS-PAGE and Agarose gel electrophoresis can both be used to separate proteins or protein- complexes…

A: Given Values: SDS-PAGE bands = 86 kD and 136 kD Agarose gel band = 222 kD and 444 kD

Q: See the attachment and answer the following parts of the question: A) If the binturong genome is…

A: Binturong is a eukaryote in which there is highly repetitive DNA sequence is present. The copies of…

Q: --TGT(G/C)CAG------3'

A: SNV means single nucleotide variants on individual RNA molecules To identify genomic differences we…

Q: A student conducted an experiment on protein isolation from mungbean. After isolating the protein,…

A: Bradford assay is an example for a general protein assay. It is used to quantify the unknown amount…

Q: 1a) In MALDI-TOF data, when performing peptide mass mapping search,  the peptide masses and the…

A: Protein identification with the help of mass spectrometry (MS) is an major step in proteomic…

Q: You add 3.0 µg of plasmid DNA to a QIAprep spin miniprep purification column. You perform the…

A: Negative charge of silanol: A silica membrane is commonly seen in QIA prep spin mini prep…

Q: You start with 100 units of protein activity and 100 grams of total protein. After the first…

A: Protein purification is performed to purify a target protein from a mixture of proteins. Fold…

Q: Estimate the time needed for the E. coli RNA polymerase at 37 °C to transcribe the entire gene for a…

A: To solve this question,  Average molecular mass of an amino acid is 110 dalton. Average rate of…

Q: 1) In MALDI-TOF data, when performing peptide mass mapping search, a) The number of peptides…

A: Protein identification with the help of mass spectrometry (MS) is an major step in proteomic…

Q: . The following data represent the base compositions of double-stranded DNA from two different…

A: A gene is the essential physical and functional unit of heredity. They are comprised of DNA…

Q: Electrophoresis 1.)a.) A protein required 6.8 min to travel 82 cm to the detector in a 96 cm -long…

A: Electrophoresis describes the separation of charged particles and their migration in the influence…

Q: How much 340bp insert DNA should be added to a ligation in which 50ng of 4.3kb vector will be used?…

A: The question is based upon the concept of molarity (number of moles of a compound per litre of…

Q: DNA intercalator and DNA alkylators are anticancer agents utilized to prevent aggressive cell…

A: Alkylating specialists substitute alkyl groups for hydrogen molecules on DNA, resulting in the…

Q: At higher amounts of protein, the Bradford assay is not linear. Consider the plot to the right: what…

A: Bradford assay is used to determine the unknown concentration of proteinbin a sample from standard…

Q: The transformation results below were obtained with 10 ul of intact plasmid DNA at nine…

A: Transformation Transformation is the process by which exogenous DNA is transferred into the host…

Q: The abundance of three mRNA transcripts, transcripts A, B, and C, was measured by real-time PCR. The…

A: In molecular biology, understanding the abundance of specific mRNA transcripts and their…

Q: Discuss the main similarities and differences in the nucleic acid sequencing methods known as…

A: DNA sequencing is the process of determining the sequence of nucleic acids, which are the basic…

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.