A full-length eukaryotic gene is inserted into a bacterial chromosome. The gene contains a complete promoter, a wildtype nucleotide sequence and a polyadenylation sequence. However, the gene does not produce a functional protein. Students may submit their answers as bullet points or a table to facilitate grading. Part A: List up to five reasons why this gene is not expressed in E. coli. Part B: What changes do you recommend that would facilitate expression of the eukaryotic gene in E. coli.
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
A full-length eukaryotic gene is inserted into a bacterial chromosome. The gene contains a complete promoter, a wildtype
Students may submit their answers as bullet points or a table to facilitate grading.
Part A: List up to five reasons why this gene is not expressed in E. coli.
Part B: What changes do you recommend that would facilitate expression of the eukaryotic gene in E. coli.
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