5. Interaction between DNA and protein forms associated complex in different biological environments and can be represented by the following equation (where D=DNA, P =Protein, and D – P = DNA-protein complex): D +P = D- P. K This association can be expressed in terms of association constant K [D-P] which is given as, K = where the terms in the parenthesis [D][P]’ represents the concentration of the species. The value of K changes NaCl Concentration when aqueous solution of NaCl is added to the system. The change of K with respect to NaCl concentration is shown in the figure. What type of interaction is predominantly present between this DNA and protein? Explain your answer.

5. Interaction between DNA and protein forms associated complex in different biological environments and can be represented by the following equation (where D=DNA, P =Protein, and D – P = DNA-protein complex): D +P = D- P. K This association can be expressed in terms of association constant K [D-P] which is given as, K = where the terms in the parenthesis [D][P]’ represents the concentration of the species. The value of K changes NaCl Concentration when aqueous solution of NaCl is added to the system. The change of K with respect to NaCl concentration is shown in the figure. What type of interaction is predominantly present between this DNA and protein? Explain your answer.

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter29: Transcription And The Regulation Of Gene Expression

Section: Chapter Questions

Problem 19P: Figure 29.15 highlights in red the DNA phosphate oxygen atoms. Some of them interact with catabolite...

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17. Mutation can be caused by the alternative base pairing that arise due to tautomerization and deamination of cysteine and adenine. Draw the alternative base pairing. The thymine dimers can be formed by UV radiation. Alternate base pairing with enol tautomer of T Alternate base pair with deaminated A Thymine dimers due to UV radn (draw structure below) repaired by the enzyme Methylated cytosine and adenine in mismatch repair
2 Suppose the following base sequence was found in a 20-base DNA polymer. 3'CAG TTA AGG CTC CTA GGT TA 5' a. What would be the first 5 bases at the 3' end of the complementary strand? а. b. What would be the first 10 bases at the 5' of the complementary strand? c. Assuming the presence of the complementary strand, what is the percentage composition of the polymer with respect to the A-T base pair and with respect to the C-G base pair? d. In the given segment, illustrate and indicate the direction of synthesis of: 1. a 5-nucleotide RNA primer 2. a 2-nucleotide Okazaki fragment
10.) Draw a double-stranded DNA molecule (using different colors for each) model should clearly represent the sequence: A G T A C C G G G C A A Note: It should include items to represent - sugar molecules - phosphate molecules - 4 distinct nitrogenous base molecules (Adenine, Thymine, Cytosine, and Guanine) - two types of bonds between these molecules
Consider normal B-form DNA. It forms a regular antiparallel double-helical structure with Watson-Crick base-pairing mediated through hydrogen bonding. The base pairs all stack upon one another, with 3.4 Å spacing between them. DNA strands having a complementary sequence will spontaneously form a double-helix in an aqueous solution. In terms of energy, what primarily drives helix formation? O Positive Entropy from base stacking van der Waals interactions O Hoogsteen interactions Positive Enthalpy from Hydrogen Bonding between GC and AT pairs Negative Enthalpy from Hydrogen Bonding between GC and AT pairs O Negative Entropy from base stacking
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2) When DNA is placed in distilled water, which is pH 7.0, it denatures (i.e., the two strands separate). The pH inside a cell is generally 7.2-7.5, depending on the organism, but DNA is generally double-stranded under physiological conditions. Briefly explain, in your own words, why DNA denatures when placed in distilled water but not when it is inside a cell. [Reminder: the pKa for the phosphate groups in the sugar-phosphate backbone of a strand of DNA is 2.14]
2 a) The following nucleotide sequence is found on the template strand of DNA: 3-TAC TGG CCG TTA GTT GAT ATA ACT-5" Determine the amino acids of the encoded peptide, then provide the altered amino acid sequence of protein that would result from each of the following mutations and provide their classification (eg. Missense, nonsense, etc). 益券 A transition at nucleotide 11 A transition at nucleotide 13 A one-nucleotide deletion at nucleotide 7 AT to A transversion at nucleotide 15 An addition of TGG after nucleotide 6 A transition at nucleotide 9
It is known that double stranded DNA is denatured at low pH. pKa values should allow the determination of whether this is due to perturbation of the hydrogen bonding in A-T and/or G-C base pairs. The table gives values for the pKas of different protonated groups in the nucleobases.Nucleobase Position & pKa A N1, 3.5 G N7, 1.6; N1, 9.2 C N3, 4.2 T N3, 9.7a) Draw the A-T and G-C base pairs. - Label the bases with the one-letter code. – - Number the atoms in the rings and label the atom that attaches to the sugar. - Mark the groups that interact in normal…
On the 4 diagrams below show the four different ways (which strands are cut and where for each Holliday junction) that the Holliday junction structures can be resolved and denote whether this leads to a recombination event or not." 3 1 IT |? 3 ||| |AL||T|||c| |||| 4 5 6 TIT!!T?L|| ?|||?| |||| TIT|| |AL||T|||C| |||| 4 5 6 3 3' 3 T?L|| ?| ||?| |!!! || |A|||T| |jcj ||| 4 5 6 TI T?L|| ?|||?T ||| jT|| |ALİ|Ti||c| ||| 4 5 6
Using the Figure below identify: What is the significance of hydrogen bonds in double helix of DN What is a role of base pairs? How genetic information might be stored in the nucleotide sequence of DNA?
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