5. Interaction between DNA and protein forms associated complex in different biological environmentsand can be represented by the following equation (where D=DNA, P=Protein, and D – P = DNA-protein complex):D +P =D – P.KThis association can be expressed in terms of association constant Kwhich is given as, K = D-P]where the terms in the parenthesis[D][P]'represents the concentration of the species. The value of K changeswhen aqueous solution of NaCl is added to the system. The change ofK with respect to NaCl concentration is shown in the figure. WhatNaCl Concentrationtype of interaction is predominantly present between this DNA and protein? Explain your answer.

Biomolecules undertake complex conjugations with each other to form complexes with unique…

Answered: 5. Interaction between DNA and protein…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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6
3. A long DNA will be divided into small fragments, and one of them will be attached to the bead and amplified in Ion Torrent sequencing. The beads with different kinds of fragments will be loaded into different wells. The following graphs show the first forty (40) signals from two different wells. The value of one (1) in the y-axis of the graph corresponds to incorporation of one (1) nucleotide to every DNA strand on a bead. (a) You can observe that in every single step the signals are not the multiples of an integer. Explain why such signals are produced and how we can interpret the signals. (b) Using the first forty (40) signals shown below, please figure out the DNA sequence of the fragment attached to the bead in each well. (c) Based on the sequences of the fragments, can we guess the partial sequence of the original DNA sequence (before fragmentation)? If so, what could it be? (d) In the well #1, when the non-zero signals will show up after the 40 steps? Answer this by the step…
1. Determine the effect of the following mutations on the DNA sequence. In each case, the mutation is described after the sequence (REFER TO THE SUPPLEMENTAL DOCUMENT FOR GUIDANCE TO THIS QUESTION). Adenine nucleotide (A shown in red below) was inserted into the DNA sequence at the position indicated by the arrow). Write out the sequence of the mutated DNA and the protein made from it. What is the effect of this mutation on the protein? (For example, how will the mutation affect the length and sequence of the protein? What about the function of the protein?) A 3' TACATGG'TTGTGCTAATT 5'
Please don't provide handwritten solution .....
17. How would you computationally predict the thermodynamic stability of a long, extended RNA helix? Please explain in 1 sentence how structural "favorability" can be predicted
8
1. What structures are these DNA strand likely to adopt in solution (assuming sufficient salt concentration to permit any hybridisation as appropriate)? Draw your answers depicting the hybridisation. a) GCC TTG AGC TTT TTT GCT CAA GGC (b) ATG ACT CTC GAG AGT CAT TTA TTA
P2. Calculate the frictional coefficient of a molecule of DNA of 20 base pairs in water at 20C; assume that the hydrodynamic behavior of the DNA itself can be approximated to be rod-like; the viscosity of the solvent is 0.01 g cm-1 s -1
7. F. State if/how Remedios' mutation changes the amino acid sequence and describe the effect that change has, if any, on the protein.
3,Compare the free energy, ∆G, of A-T binding and A-C binding, which one is more negative? Does this makes sense in terms of the structure of DNA? Group of answer choices A, ∆G of the A-T binding is more negative than that of the A-C binding, which is consistent with the fact that A-C binding is not favored in DNA helix. B, ∆G of the A-T binding is more negative than that of the A-C binding, which is consistent with the fact that A-C binding is favored in DNA helix. C, ∆G of the A-C binding is more negative than that of the A-T binding, which is consistent with the fact that A-C binding is not favored in DNA helix. D, ∆G of the A-C binding is more negative than that of the A-T binding, which is consistent with the fact that A-C binding is favored over A-T binding in DNA helix.
Which kind of chemical damage is depicted in this picture: 2-OH Ostrand breakage Obase deletion Obase modification O base cross linking is in
2. pBR3322, an artificially made plasmid, is shown below. ECORI Hindill BamHI Pstl Amp ORI ↓↓ Tetr pBR322 (4.36 kb) ↑ Pvull Xmalll Describe 3 features that this plasmid has and indicate each one on the diagram. i) ii) 111]

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