2. The pathways for the biosynthesis of the amino acidsglutamine (Gln) and proline (Pro) involve one or morecommon intermediates. Auxotrophic yeast mutantsnumbered 1–7 are isolated that require either glutamine or proline or both amino acids for their growth,as shown in the following table (+ means growth; −no growth). These mutants are also tested for theirability to grow on the intermediates A–E. What is theorder of these intermediates in the glutamine and proline pathways, and at which point in the pathways iseach mutant blocked?Gln +Mutant A B C D E Gln Pro Pro1 + − − − + − + +2 − − − − − − + +3 − − + − − − − +4 − − − − − + − +5 − − + + − − − +6 + − − − − − + +7 − + − − − + − +

Human Anatomy & Physiology (11th Edition)
11th Edition
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Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
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2. The pathways for the biosynthesis of the amino acids
glutamine (Gln) and proline (Pro) involve one or more
common intermediates. Auxotrophic yeast mutants
numbered 1–7 are isolated that require either glutamine or proline or both amino acids for their growth,
as shown in the following table (+ means growth; −
no growth). These mutants are also tested for their
ability to grow on the intermediates A–E. What is the
order of these intermediates in the glutamine and proline pathways, and at which point in the pathways is
each mutant blocked?
Gln +
Mutant A B C D E Gln Pro Pro
1 + − − − + − + +
2 − − − − − − + +
3 − − + − − − − +
4 − − − − − + − +
5 − − + + − − − +
6 + − − − − − + +
7 − + − − − + − +

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