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Q15) The pathway below is an anabolic pathway, where it is thought that products D and E are essential for growth, but F is not. You have strains containing mutations in one of each of the enzymes (1, 2, 3, 4, 5) known to be involved in the pathway. You grow each strain with the addition of different intermediates and assess their ability to grow. E 4 3. A 1 a) Predict the results you would expect in the table below. “+" means that when a strain containing a mutation in the enzyme on the left is incubated in the presence of the metabolite at the top of the column, the strain is able to grow. “-" means the strain containing that mutation fails to grow in media in the presence of the metabolite at the top of the column. C E F 1 3 4 b) When you carry out the experiment, you are surprised to find that supplementing with excess F causes all the strains to stop growing, unless they are also supplemented with B. Suggest an explanation for this finding.

Q15) The pathway below is an anabolic pathway, where it is thought that products D and E are essential for growth, but F is not. You have strains containing mutations in one of each of the enzymes (1, 2, 3, 4, 5) known to be involved in the pathway. You grow each strain with the addition of different intermediates and assess their ability to grow. E 4 3. A 1 a) Predict the results you would expect in the table below. “+" means that when a strain containing a mutation in the enzyme on the left is incubated in the presence of the metabolite at the top of the column, the strain is able to grow. “-" means the strain containing that mutation fails to grow in media in the presence of the metabolite at the top of the column. C E F 1 3 4 b) When you carry out the experiment, you are surprised to find that supplementing with excess F causes all the strains to stop growing, unless they are also supplemented with B. Suggest an explanation for this finding.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Q15) The pathway below is an anabolic pathway, where it is thought that products D and E are essential for growth, but F is not. You have strains containing mutations in one of each of the enzymes (1, 2, 3, 4, 5) known to be involved in the pathway. You grow each strain with the addition of different intermediates and assess their ability to grow. E D 4 3 A 1 a) Predict the results you would expect in the table below. “t" means that when a strain containing a mutation in the enzyme on the left is incubated in the presence of the metabolite at the top of the column, the strain is able to grow. containing that mutation fails to grow in media in the presence of the metabolite at the top of the column. means the strain В C D E F 1 3 4 5 b) When you carry out the experiment, you are surprised to find that supplementing with excess F causes all the strains to stop growing, unless they are also supplemented with B. Suggest an explanation for this finding.
Transcribed Image Text:Q15) The pathway below is an anabolic pathway, where it is thought that products D and E are essential for growth, but F is not. You have strains containing mutations in one of each of the enzymes (1, 2, 3, 4, 5) known to be involved in the pathway. You grow each strain with the addition of different intermediates and assess their ability to grow. E D 4 3 A 1 a) Predict the results you would expect in the table below. “t" means that when a strain containing a mutation in the enzyme on the left is incubated in the presence of the metabolite at the top of the column, the strain is able to grow. containing that mutation fails to grow in media in the presence of the metabolite at the top of the column. means the strain В C D E F 1 3 4 5 b) When you carry out the experiment, you are surprised to find that supplementing with excess F causes all the strains to stop growing, unless they are also supplemented with B. Suggest an explanation for this finding.
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