(4521) Ahdl (3628) Peit (1001) Scal (4801) Pul 4000 (3249) Bt2171 (3197) Bam (5325) Sep 5000 Ampl (3052) Pol SV40 poly(A) signal (2959) BB (5442) gr O streptavidin column O anti-myc column o hydrophobic column ○ Ni²+-NTA column AmpR promoter 3000 Bill (12) (2793) R 5444 bp NeoR/Kank Mel (161) Bpu101 (180) Nrul (20) Mul (228) Spel (249) Ndel (44) CMV enhancer CMV promoter BHII (2674) SV40 on 17 prom er | MCS 5GH poly(A) signa O OPAS 0002 Snabl (590) for Nhel (95) Bmt (199) Kast (2276) Pmel (904) ANI (908) Hind SexAI (1834) BRI (2063) Stul (2066) Avril (2067) TspMI-Xmal (2008) Smal (2090) BsaB (2136) Nart (2277) Stol (2278) PluT! (2290) Mack (2359) PAFI Th1111 (295) (911) Berest (17) (921) Kont BamHI (29) EcoRI (935) EcoRV (147) BEXI (957) Not! (962) PaeR71 -PspXI-Xhol (965 Xbal (974) Eco01091 PspOMI (960) Apal (984) Myc Bbs (1230) Drall (1544)
(4521) Ahdl (3628) Peit (1001) Scal (4801) Pul 4000 (3249) Bt2171 (3197) Bam (5325) Sep 5000 Ampl (3052) Pol SV40 poly(A) signal (2959) BB (5442) gr O streptavidin column O anti-myc column o hydrophobic column ○ Ni²+-NTA column AmpR promoter 3000 Bill (12) (2793) R 5444 bp NeoR/Kank Mel (161) Bpu101 (180) Nrul (20) Mul (228) Spel (249) Ndel (44) CMV enhancer CMV promoter BHII (2674) SV40 on 17 prom er | MCS 5GH poly(A) signa O OPAS 0002 Snabl (590) for Nhel (95) Bmt (199) Kast (2276) Pmel (904) ANI (908) Hind SexAI (1834) BRI (2063) Stul (2066) Avril (2067) TspMI-Xmal (2008) Smal (2090) BsaB (2136) Nart (2277) Stol (2278) PluT! (2290) Mack (2359) PAFI Th1111 (295) (911) Berest (17) (921) Kont BamHI (29) EcoRI (935) EcoRV (147) BEXI (957) Not! (962) PaeR71 -PspXI-Xhol (965 Xbal (974) Eco01091 PspOMI (960) Apal (984) Myc Bbs (1230) Drall (1544)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Concept explainers
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
Question
Transcribed Image Text:What column can be used to purify a protein produced using the vector below?
(4521) AhdI
(3628) Pil
(5001) Scal
(4891) Pul
£4000
(3249) BstZ171
(3197) Baml
(5325) Sept
5000
AmpR
(3012) Pol
(5442) SgrDI
SV40 poly(A) signal
(2959) BIBI
O streptavidin column
O anti-myc column
O hydrophobic column
○ Ni²+-NTA column
IK
AmpR promoter
3000
Ball (12)
(2793) Rsrl
5444 bp
Mel (161)
Bpu101 (180)
Nrul (20)
Mlul (228)
CMV
NeoR/Kank
chancer Sar
CMV promromot
BsSHII (2674)
ear M
SV40 or
n
n
24
Spel (249)
Nel (484)
17)
1000
bGH po
promote
SnaBI (590)
Nhel (895)
Bmtl (899)
poly(A) signal
C0027
ori
PluTI (2290)
MsCI (2359)
PRIFI- Th1111 (2395)
Created with SnapGene
Pmel (904)
AMII (908)
HindIII (911)
Arch11 (917)
Kpnl (921)
BamHI (929)
EcoRI (935)
EcoRV
(947)
BetXI (957)
Not! (962)
Kast (2276)
Nart (2277)
Stol (2278)
PaeR71 - PspXI - XhoI (968)
PspOMI (960)
Xbal (974)
Eco01091
Apal (984)
Myc
Bbs (1230)
Drall (1544)
-BseRI (2053)
Stul (2066)
AvrII (2067)
TspMI Xmal (2008)
Smal (2090)
BsaB1 (2136)
SexAI (1834)
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