Spliceosome assembly and function AG GU Exon 5' AG GU Exon Intron SNPS U1 and U2 bind to the 5' splice site and internal A U1 U2 U1 AG GU U2 AG GU The U4-U5-U6 U4 U5 complex joins the U6 spliceosome U4 AGGU U6 AG GU U5 U4) U1 U6 First splicing reaction: one intron end attaches to A |Second splicing reaction: other intron end cleaved; exons join AGIGU Spliced exons FIGURE 8-16 The spliceosome is composed of several snANPs that attach sequentially to the RNA, taking up positions roughly as shown. Alignment of the snRNPs results from hydrogen bonding of their snRNA molecules to the complementary sequences of the intron. In this way, the reactants are properly aligned and the two splicing reactions can take place. The chemistry of these reactions can be seen in more detail in Figure 8-17. Reactions in exon splicing Intron но 5' O=P- O- Exon 1 5 Exon 2 First transesterification 5' 2' O-P-O -0-- 3' Second transesterification 5' 2 Spliced exons 3' он Excised lariat intron 0 = 3' oxygen of exon 1 0 = 2' oxygen of branch point A 0= 3' oxygen of intron FIGURE 8-17 Two transesterification reactions take place in the splicing of RNA: first, to join the 5' donor end of the intron to the internal branch point (first reaction in Figure 8-16) and, second, to join the two exons together (second reaction in Figure 8-16).
Spliceosome assembly and function AG GU Exon 5' AG GU Exon Intron SNPS U1 and U2 bind to the 5' splice site and internal A U1 U2 U1 AG GU U2 AG GU The U4-U5-U6 U4 U5 complex joins the U6 spliceosome U4 AGGU U6 AG GU U5 U4) U1 U6 First splicing reaction: one intron end attaches to A |Second splicing reaction: other intron end cleaved; exons join AGIGU Spliced exons FIGURE 8-16 The spliceosome is composed of several snANPs that attach sequentially to the RNA, taking up positions roughly as shown. Alignment of the snRNPs results from hydrogen bonding of their snRNA molecules to the complementary sequences of the intron. In this way, the reactants are properly aligned and the two splicing reactions can take place. The chemistry of these reactions can be seen in more detail in Figure 8-17. Reactions in exon splicing Intron но 5' O=P- O- Exon 1 5 Exon 2 First transesterification 5' 2' O-P-O -0-- 3' Second transesterification 5' 2 Spliced exons 3' он Excised lariat intron 0 = 3' oxygen of exon 1 0 = 2' oxygen of branch point A 0= 3' oxygen of intron FIGURE 8-17 Two transesterification reactions take place in the splicing of RNA: first, to join the 5' donor end of the intron to the internal branch point (first reaction in Figure 8-16) and, second, to join the two exons together (second reaction in Figure 8-16).
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Proteins
We generally tend to think of proteins only from a dietary lens, as a component of what we eat. However, they are among the most important and abundant organic macromolecules in the human body, with diverse structures and functions. Every cell contains thousands and thousands of proteins, each with specific functions. Some help in the formation of cellular membrane or walls, some help the cell to move, others act as messages or signals and flow seamlessly from one cell to another, carrying information.
Protein Expression
The method by which living organisms synthesize proteins and further modify and regulate them is called protein expression. Protein expression plays a significant role in several types of research and is highly utilized in molecular biology, biochemistry, and protein research laboratories.
Question
By comparing Figures 8-16 and 8-17, evaluate what is/are
the function(s) of proteins U1–U6.
Transcribed Image Text:Spliceosome assembly
and function
AG GU
Exon
5'
AG GU
Exon
Intron
SNPS U1 and U2
bind to the 5' splice
site and internal A
U1 U2
U1
AG GU
U2
AG GU
The U4-U5-U6
U4 U5
complex joins the
U6
spliceosome
U4
AGGU U6
AG GU
U5
U4) U1
U6
First splicing
reaction: one intron
end attaches to A
|Second splicing
reaction: other intron
end cleaved;
exons join
AGIGU
Spliced exons
FIGURE 8-16 The spliceosome is
composed of several snANPs that attach
sequentially to the RNA, taking up
positions roughly as shown. Alignment of
the snRNPs results from hydrogen
bonding of their snRNA molecules to the
complementary sequences of the intron. In
this way, the reactants are properly aligned
and the two splicing reactions can take
place. The chemistry of these reactions
can be seen in more detail in Figure 8-17.
Transcribed Image Text:Reactions in exon splicing
Intron
но
5'
O=P-
O-
Exon 1
5
Exon 2
First transesterification
5'
2'
O-P-O
-0--
3'
Second transesterification
5'
2
Spliced exons
3'
он
Excised lariat intron
0 = 3' oxygen of exon 1
0 = 2' oxygen of branch point A
0= 3' oxygen of intron
FIGURE 8-17 Two transesterification reactions take place in the splicing of RNA: first, to
join the 5' donor end of the intron to the internal branch point (first reaction in Figure 8-16)
and, second, to join the two exons together (second reaction in Figure 8-16).
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