If you have the same protein loaded in two different lanes of an SDS-PAGE stained with Coomasie blue, and one lane has a darker, thicker band of protein than the other lane, can you say that there is more protein in the lane with the darker band? Why or why not?

Q: This gel has approximately 10mg of protein in each lane.  * What would this gel look like if you…

A: A particular type of gel has a specific sensitivity, SDS-PAGE gels have a high sensitivity that 2ug…

Q: If the target protein is 0.1% of the total protein in the original mixture, a three-step…

A: Purification is a process by which impurities are removed from a sample and desired component is…

Q: Proteinase K should be used as the first step of DNA purification (before applying phenol CIA…

A: Proteins are large, complex molecules made up of chains of amino acids that perform various…

Q: It is maintained by a bond with resonance structures. It will be disrupted by extreme pH conditions.…

A: A protein's biological function depends on its three-dimensional structure. The 3D structure is…

Q: , calculate the average amino acid residue weight in a protein of typical composition. This is a…

A: Proteins are composed of the linear chain of amino acids attached via peptide bonds. Proteins are…

Q: In order to prepare a standard curve, you will do serial dilutions of the standard protein. Assume…

A: Serial dilutionsIt refers to the stepwise dilution of a solution to create a series of solutions…

Q: After a polypeptide chain has been synthesized, certain amino acids in the peptide may become…

A: The modified amino acid shown in the structure you provided is serine (Ser).The modification that…

Q: Why would your protein measurement not match to package label? Let us disregard the idea that the…

A: One plausible explanation for a discrepancy between the measured protein content of a product and…

Q: As an example, consider albumin, a protein made of a single polypeptide weighing 66,000 daltons (66…

A: Gel electrophoresis if the technique of separation of molecules on the basis of their size and…

Q: You are purifying a protein for the first me and decide to analyze it on SDS PAGE. You run two lanes…

A: A. This finding implies that the protein likely has disulfide links in its natural state. Disulfide…

Q: In a folded protein, Glu116 is close in three-dimensional space to Lys224. The pKa of the carboxylic…

A: pKa is the pH at which a weak acid is 50% dissociated. Alternatively, pKa = -log Ka. In acid-base…

Q: small protein has a sequence ACNCKAAPMLCARYCAMLH. The structure of this single peptide is stabilized…

A:

Q: Trypsin digestion of the peptide sequence M3-E4-R5-P6-F7-Hg-Ag-10-A11-R12-T13-Y14- G15-P16 results…

A:

Q: In TLC, why are the colors of the peaks for each amino acid are different depending on the types of…

A: TLC stands for Thin Layer Chromatography. It is a refined technique that is used for isolating or…

Q: You need to resuspend lyophilized protein in a solution of 5mM HEPES with 20% glycerol. Describe how…

A: Resuspending lyophilized proteins in buffer solutions is basic in numerous biochemical and…

Q: Given the following information, which protein would be most difficult to isolate from protein X…

A: Salting and dialysis are the processes used to isolate proteins. Salting is a purification process…

Q: titrated amino acids appear to be acidic, basic, or neutral amino acids

A: Biomolecules that form proteins are called amino acids. An amino acid consists of a basic amino…

Q: What kinds of changes take place in a protein when it is denatured?

A: Denaturation is a process in which a protein loose the quaternary structure, tertiary structure, and…

Q: Transthyretin is a large protein with 508 amino acids. How much in daltons would one exoect this…

A: Transthyretin is transport protein that transports thyroid hormone.

Q: Using ultrafiltration, you concentrate a solution of Protein X as follows: Initial volume protein X…

A: Ultrafiltration is a laboratory technique used to remove minute-level impurities from biological…

Q: Which amino acid pair constitutes the hydrophobic patch on sickle cell hemoglobin that results in…

A: Sickle cell disease is caused due to inversion of one base pair (A -> T). Due to which, the sixth…

Q: HOW MANY of the following five items represent modes of protein denaturation? 1.) heating a protein…

A: Proteins are biomolecules composed of amino acids. Amino acids are joined together through peptide…

Q: A protein has a molecular mass of 400 kDa when measured by size-exclusion chromatography. When…

A: Size exclusion chromatography is a technique which separates molecules according to their size…

Q: otal protein is used.

A: Proteins can be defined as large biomolecules that consist of one or more long chains of amino acid…

Q: Provide a brief definition for a polymorphic protein

A: Polymorphism means have many types of morphology and structure. It is a condition when more than one…

Q: Proteins are essential to most processes in the human body. Given your understanding of the M phase…

A: Cell Cycle and Phases Of Cell Cycle ---- The ordered sequences of events which takes place in the…

Q: Jacqueline isolated an interesting new protein from the broth of a yeast culture. After purification…

A: The minimum molecular weight of the isolated protein was calculated as                Residue% =…

Q: A spheroidal bacterium with a diameter of 1.0 mm (micrometer, 1 mm = 10-6) contains 25,000 molecules…

A: A spheroidal bacterium are circular or oval in shape which are generally known as coccus (plural…

Q: Which of the following amino acids would you expect to find more often near the center of a folded…

A: Proteins are biomolecules made up of long chains of amino acids. It is macronutrient that is…

Q: The limit for G-200 beads is 5000-600,000. When you pass two proteins- Protein A (75, 000), Protein…

A: Proteins are amazing molecules that make everything simple inside the cell. Protein molecules are…

Q: Serum Albumin (Monomer) Ovalbumin 66,000 43,000 32mm 39mm 60mm 26mm 14,400 Lysozyme Unknown 1 ?…

A: Distance Molecular weight 23 132000   32 66000   39 43000   60 14400…

Q: The PAM1 matrix penalizes mismatching amino acids more than higher numbered PAM matrices. Explain…

A: PAM (POINT ACCEPTED MATRIX) indicates the probability of a mutation of an amino acid in a given…

Q: A protein is likely to become a denatured when?

A: Proteins are amino acids residues joined together by peptide bonds , which form extremely complex…

Q: What are the different biologic functions of protein? Give at least eight (8) and elaborate on each…

A: Asked : 8 biological function of proteins

Q: Assume that the 3 polypeptide strands shown below form a parallel B-sheet. Select amino acids AA1,…

A: Beta sheet is one of type of secondary structure in which inter strand hydrogen bonds is formed…

Q: You want to isolate a protein which is thermally highly stable. This protein is currently in…

A: Proteins are defined as the large and complex molecules that are formed by one or more than one…

Q: KDa 97.4 66.24 45.0 31.0 21.5 14.4 8-1 1-d s-2 For condition 1 - For condition 2 - For condition 3 -…

A: This question is asking about the dissemination of proteins within the supernatant and pellet…

Q: You gathered your data from Lab 4, and found the following values: Total Activity of lysozyme in…

A: Total Activity of lysozyme in HEW: 14 units  Total amount of protein in HEW: 70 mg  Total Activity…

Q: You have a protein with MW 48kDa. How many grams of protein are in a 200 mL sample at 50 μM?

A: To calculate the amount of protein in grams in a 200 mL sample with a concentration of 50 μM, follow…

Q: More specifically, the component monosaccharides are (D-galactose, L-galactose), (D-glucose,…

A: Here the two matters of concern are: 1)  L and D isomers: Depending on the position of the –OH group…

Q: Explain Mosaic analysis determines the focus of action of Sevenless protein.

A: The recombination between genes takes place during meiosis that leads to the production of gametes.…

Q: The following fragments are produced from a trypsin digest: LPNVSDFTMDCEWQASCR              TYILHFN…

A: Proteins are unbranched polymers constructed from 20 standard α-amino acids. They have four…

Q: A protein was purified and then analyzed on a size exclusion column. Its MW was determined to be…

A: SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis) is used for separating proteins…

Q: A particular amino acid contains a -CH2NH3+ group.  Is this amino acid more likely to be found on…

A: In a folded globular protein charged and polar amino acids on the surface of the protein are…

Q: What is one technique or property of a protein that you could use to monitor the fractions so you…

A: In column chromatography, there is a stationary phase and the mobile phase. The stationary phase…

Q: Proteoglycans are often made up of glycosaminoglycans (chondroitin sulfate, keratan sulfate, and…

A: On the basis of composition, proteins can be either simple or conjugated. Conjugated proteins…

Q: What contributes to the asymmetric protein distributions ?

A: The lipid bilayer (or phospholipid bilayer) is a thin polar membrane made of two layers of lipid…

Q: Identify 10 protein-containing materials that can be found in a household. Identify the specific…

A: Protein play wide variety of essential function in our body. They provide strength and structural…

Microbial Taxonomy

Taxonomy is the science of naming, classifying, and describing organisms. The taxonomy will include all the plants, microorganisms, and animals on this planet. Carl Linnaeus coined the taxonomy term. He is known as the father of taxonomy. Microbial taxonomy is a criterion of grouping microorganisms into groups based on their similarities and past relatedness. Microorganisms belonging to the same group are differentiated from the others via grouping into categories. This differentiation makes the study of microorganisms easy. There are different types of microbial taxonomies like Numerical and Molecular taxonomy. The study of microorganisms is known as microbiology. Microbiology is a broad term that takes into account microbial cultures, taxonomic studies, evolutionary relationships. Microbial interaction and diversity are huge data that needs to be compiled under the subject of microbiology. Microbiology covers various organisms like bacteria, fungi, algae, plants, animals, etc.