If you have the same protein loaded in two different lanes of an SDS-PAGE stained with Coomasie blue, and one lane has a darker, thicker band of protein than the other lane, can you say that there is more protein in the lane with the darker band? Why or why not?

Q: This gel has approximately 10mg of protein in each lane.  * What would this gel look like if you…

A: A particular type of gel has a specific sensitivity, SDS-PAGE gels have a high sensitivity that 2ug…

Q: Why would your protein measurement not match to package label? Let us disregard the idea that the…

A: One plausible explanation for a discrepancy between the measured protein content of a product and…

Q: As an example, consider albumin, a protein made of a single polypeptide weighing 66,000 daltons (66…

A: Gel electrophoresis if the technique of separation of molecules on the basis of their size and…

Q: In an experiment of egg white and calamansi juice in protein denaturation, what will happen?

A: Proteins are one of the  most important metabolites in living organisms, which is almost present in…

Q: In a folded protein, Glu116 is close in three-dimensional space to Lys224. The pKa of the carboxylic…

A: pKa is the pH at which a weak acid is 50% dissociated. Alternatively, pKa = -log Ka. In acid-base…

Q: Disulfide bonds have been shown to stabilize proteins (i.e., make them less likely to unfold).…

A: Disulfide bonds are considered the covalent bond, which is formed between the two cysteine residues.…

Q: Is the product SARDINES is affected by protein denaturation during processing and storage? If so, is…

A: Protein denaturation is a concern in fisheries products. As a result, additional verification tests…

Q: Are there anymore features that limit the protein configurations?

A: A protein's biological function depends on its three-dimensional structure. The 3D structure is…

Q: The 20 different amino acids in the protein are normally coded by ------ nucleotides. * 60 61 62 63

A: Nucleotides are building blocks of nucleic acids. Three mRNA nucleotides present together are known…

Q: List the 4 types of protein modification and describe each in 1 sentence

A: Protein modification refer to any change in the chemical composition of proteins following…

Q: In the amino acid sequence below, the amino acid residue in red was shown to be essential to the…

A: Peptides or proteins are composed of twenty standard amino acids. These standard amino acids differ…

Q: What are the four steps for the production of a protein?  What happens in each of these steps that…

A: Protein biosynthesis is a center natural interaction, happening inside cells, adjusting the…

Q: You are trying to purify Protein 1 from a mix of 4 different proteins without denaturing the…

A: Ion exchange chromatography is a techniques that separates ions and polar molecules based on their…

Q: 80 20 60 0 60 20

A: It is a way to visualise angles as a function of angles . It is the way we could identify the…

Q: A polypeptide with a pH of 2.0 has a total net charge of 0. Gly-Pro-Glu-Asp-Leu-His-Ile-Gln-Asn-Phe…

A: This peptide is composed of glycine, proline, glutamic acid, aspartic acid, leucine, histidine,…

Q: Can someone give me examples of denatured proteins that can be brought back to native state…

A: The question asks the examples of denatured protein which can be transformed into the renatured or…

Q: Transthyretin is a large protein with 508 amino acids. How much in daltons would one exoect this…

A: Transthyretin is transport protein that transports thyroid hormone.

Q: You are trying to isolate a protein you don't know much about, so you decide to use a gel column…

A: Introduction :-  Chromatography is a biophysical technique which is used to separate or isolate the…

Q: What is the importance of this protein?? Please explain.

A: Protein is the foundation to every human body, required by every cell in the body, and is an…

Q: This is a picture of an SDS-PAGE gel. In the left lane are protein standards with molecular masses…

A: SDS-PAGE is used determining the molecular weight (MW) of an unknown protein. The first step the…

Q: imagine that you are running a sample on a TLC plate that is comprised of aspartate and isoleucine.…

A: The working principle of thin-layer chromatography is the same as column chromatography.A small…

Q: Which amino acid pair constitutes the hydrophobic patch on sickle cell hemoglobin that results in…

A: Sickle cell disease is caused due to inversion of one base pair (A -> T). Due to which, the sixth…

Q: That's okay; you're just stoked to be doing international collaborative research     That's okay;…

A: Deoxy ribonucleic acid (DNA) is the genetic material of most organisms that carry coded genetic…

Q: Which amino acid is classified as neutral and non-polar?

A: Amino acids when joined together by a peptide bond synthesizes the protein molecules. Amino acids…

Q: Give an example of a protein containing primarily betapleated sheets. Is this a fibrous or globular…

A: Protein structure decides the amino acid sequence, chemical bonds found between atoms in the…

Q: what are the structural features of the protein 6VJI? primary , secondary What are two features of…

A: Amino acids are the building blocks of proteins as numerous amino acids joined to one another…

Q: What protein purification methods can be used to ensure that the resulting protein is soluble ?

A: The method used for protein purification that can be used to ensure that the resulting protein is…

Q: otal protein is used.

A: Proteins can be defined as large biomolecules that consist of one or more long chains of amino acid…

Q: Proteins are essential to most processes in the human body. Given your understanding of the M phase…

A: Cell Cycle and Phases Of Cell Cycle ---- The ordered sequences of events which takes place in the…

Q: Explain the difference between essential and nonessential amino acids. How many of each are there?…

A: Proteins are one of the macronutrients present in the food. They are very essential for growth and…

Q: What is the protein product of the following DNA sequence? 5'-AACTCCGACTACGCTCCATTTCGAGCA-3'…

A: Proteins are complex biomolecules made up of chains of amino acids that fold into specific…

Q: What are the 2 main categories of proteins? B. What are the features of protein in these categories

A: As per the central dogma of biology, the genetic information stored within the DNA is transcribed…

Q: A gel filtration column with a fractionation range of 1.5-20 kDa is used to separate out the…

A: Gel filtration chromatography is a technique that is used to separate molecules based on their…

Q: The limit for G-200 beads is 5000-600,000. When you pass two proteins- Protein A (75, 000), Protein…

A: Proteins are amazing molecules that make everything simple inside the cell. Protein molecules are…

Q: Serum Albumin (Monomer) Ovalbumin 66,000 43,000 32mm 39mm 60mm 26mm 14,400 Lysozyme Unknown 1 ?…

A: Distance Molecular weight 23 132000   32 66000   39 43000   60 14400…

Q: Proteins can be separated into 9 general classifications based on the role they play in a cell. List…

A: Proteins can be classified into following types:- Fibrous Proteins Globular Proteins Derived…

Q: What are other substances/situations that can cause protein precipitation?

A: Introduction: Proteins are nitrogenous macromolecules that are the polymers of amino acids. It…

Q: Why is denaturing the proteins necessary for SDS PAGE to work? Use YOUR OWN BERITEL

A: Introduction  Proteins are large, complex molecules that perform a wide range of essential functions…

Q: Identify a disease or condition related to protein mis-folding.  Which protein is misfolded?  Is…

A: Accumulation of misfolded proteins cause disease various neurodegenerative diseases, such as amyloid…

Q: Find a method that uses some form of HPLC for the analysis of proteins. What was the stationary…

A: Protein analysis by chromatography is a widely used technique for separating, identifying, and…

Q: Upon doing the experiment of Protein Denaturation, what could happen in the precipitation of strong…

A: Denaturation is the disruption and unfolding of a protein, resulting in the loss of its biological…

Q: Erk and P-Erk live in two different locations within the cell even though they are just two…

A: * Mitogen activated protein kinase also called as  MAPK and  p42MAPK, and ERK2 * It is an enzyme…

Q: In the isolation of protein casein from milk, why is heated distilled water added into the milk,…

A: Casein is an abundant protein of milk which is globular in structure.It has iso electric point of…

Q: GC-MS can be used to separate and identify proteins, and HPLC is commonly used to separate lipids…

A: GC-MS can be used to separate and identify proteins, and HPLC is commonly used to separate lipids it…

Microbial Taxonomy

Taxonomy is the science of naming, classifying, and describing organisms. The taxonomy will include all the plants, microorganisms, and animals on this planet. Carl Linnaeus coined the taxonomy term. He is known as the father of taxonomy. Microbial taxonomy is a criterion of grouping microorganisms into groups based on their similarities and past relatedness. Microorganisms belonging to the same group are differentiated from the others via grouping into categories. This differentiation makes the study of microorganisms easy. There are different types of microbial taxonomies like Numerical and Molecular taxonomy. The study of microorganisms is known as microbiology. Microbiology is a broad term that takes into account microbial cultures, taxonomic studies, evolutionary relationships. Microbial interaction and diversity are huge data that needs to be compiled under the subject of microbiology. Microbiology covers various organisms like bacteria, fungi, algae, plants, animals, etc.