If you have the same protein loaded in two different lanes of an SDS-PAGE stained with Coomasie blue, and one lane has a darker, thicker band of protein than the other lane, can you say that there is more protein in the lane with the darker band? Why or why not?

Q: Why is the onion mixture processed through the blender?

A: DNA extraction is the process of isolating  DNA molecule from a biological sample. This was first…

Q: expanded structure of the D-tagatose Fischer projection of D-tagatose Haworth projection of…

A: The objective of the question is to understand the different structural representations of…

Q: How does the width of the black iodide starch complex of each potato block compare to the others?…

A: lodine solution is basically acting as an indicator for starch solution which is there in potato.…

Q: The rate of migration of nucleic acid fragments through an agarose gel is determined by the_

A: The gel electrophoresis is used to separate DNA fragments based on their size, the gel…

Q: It is known that 80% of Penicillin is protein bound. Explain how most of it is being cleared from…

A: Penicillin is an antibiotic and a beta lactam drug. Penicillin upon administration into humans , go…

Q: 63. Given the single stranded DNA shown below (written 5' to 3') and the genetic code, which…

A: As per the central dogma of molecular biology, genetic information is stored in the DNA. The genetic…

Q: A solution of peptide of unknown sequence was divided into 2 samples. One sample was tre with…

A: Site specific proteases are enzymes that cleaves polypeptide chains only at specific sites i.e. at…

Q: How do you prepare 50 ml of 20 mg/ml proteinase K from 50 mg/ml stock solution

A: Proteinase K is a serine proteinase enzyme used to digest proteins. It is produced from a…

Q: Are all the proteins separated properly using SDS-PAGE? Why or why not?

A: Proteins are massive biomolecules and macromolecules made up of one or more long chains of amino…

Q: Why does the violet color of the Hubble’s reagent fade away in unsaturated fats/oil?

A: a. Fatty acids are classified into saturated and unsaturated fatty acids. b. Saturated fatty acids…

Q: The order is for Moxam 5 gm IM every 8 hours.  On hand is 10 gram of Moxam with the following…

A: Medication order is the terms used to define the directions for a drug needed to be given to the…

Q: Why would your protein measurement not match to package label? Let us disregard the idea that the…

A: One plausible explanation for a discrepancy between the measured protein content of a product and…

Q: what is the difference between glucosidic linkage and glycosidic lingake

A: These are a number of types of bonds that can lie between different biological compounds. Some…

Q: Amino acid sequence analysis of all of the peptides found in a single IgG antibody would reveal…

A: Antibodies are made of immunoglobulin (Ig) units. They are associated with the immune system and are…

Q: what is not an aminoglycoside?

A: Aminoglycosides are a class of anti-infection agents utilized widely in the treatment of aerobic…

Q: In a folded protein, Glu116 is close in three-dimensional space to Lys224. The pKa of the carboxylic…

A: pKa is the pH at which a weak acid is 50% dissociated. Alternatively, pKa = -log Ka. In acid-base…

Q: Disulfide bonds have been shown to stabilize proteins (i.e., make them less likely to unfold).…

A: Disulfide bonds are considered the covalent bond, which is formed between the two cysteine residues.…

Q: What is Norit? What is it made of? What is it used for?

A: 1.What is Norit..... Ans. Norit is a Activated carbon, also called activated charcoal, is a form of…

Q: In DNA double digestion, what does star activity mean and why should it be considered in choosing…

A: Restriction digestion refers to the process of digestion of DNA strand(s) with the help of specific…

Q: What is BP ? My BP is 100/150 . is it good or bad for my health ?

A: KEY WORDS :- BP - Blood pressure. Normal blood pressure - 120/80 MMHG.

Q: A very common molecular biology research method is to analyze cell or tissue homogenates by…

A: In molecular biology research, SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting,…

Q: Draw the Gentiobiose: 2 Glucose, beta-(1,6)

A: Gentiobiose is a disaccharide composed of two subints of D-glucose via β(1-6) linkage. It is soluble…

Q: Analysis of a 49kDA protein is aimed with a western blot technique. For this purpose, "whole cell…

A: Western blot is a technique in which the concentration of a protein is determined using SDS PAGE…

Q: Stapphylococcus aureus V8 protease cleaves at the C-termini of Asp and Glu. How many fragments do…

A: Protein is a sequence of amino acid which are joined by peptide bonds.

Q: What is Folate Trap ? Explain the importance of Folate Trap ?

A: In a human body due to deficiency of vitamin B12 folic acid is rendered inactive this phenomenon is…

Q: The following list of proteins are found in a mixture and are to be separated by 2D gel…

A: In 2D gel electrophoresis, is a 2 step-based separation of protein.  In the first dimension, the…

Q: You are trying to isolate a protein you don't know much about, so you decide to use a gel column…

A: Introduction :-  Chromatography is a biophysical technique which is used to separate or isolate the…

Q: A. In replicate analyses, the carbohydrate content of a glycoprotein ( a protein with sugars…

A: According to the given data, the glycoprotein having 11.9g of carbohydrate content in 100g of…

Q: That's okay; you're just stoked to be doing international collaborative research     That's okay;…

A: Deoxy ribonucleic acid (DNA) is the genetic material of most organisms that carry coded genetic…

Q: You will have to dilute your inital Lysozyme stock in order to pipet volumes larger than 10 uL for…

A: Step 1:For the Bradford assay, the typical concentration range of protein you want to target is…

Q: Why is rye flour considered as a non-wheat flour?

A: Rye is a hardly cereal grass cultivated for its grain. Both wheat and rye are highly nutritious.

Q: A student conducted an experiment on protein isolation from mungbean. After isolating the protein,…

A: Bradford assay is an example for a general protein assay. It is used to quantify the unknown amount…

Q: You have been given an unknown low weight polypeptide to sequence. Luckily, you have a plentiful…

A: Proteins are composed of amino acids, which are bound together by peptide linkage. Amino acids…

Q: What is the single definitive test for galactose? State the principle.

A: Galactose is a monosaccharide . it combines with glucose to form a disaccharide called lactose. It…

Q: You start with 100 units of protein activity and 100 grams of total protein. After the first…

A: Protein purification is performed to purify a target protein from a mixture of proteins. Fold…

Q: what does it mean to be lactose intolerant?

A: Lactose is a natural sugar found in milk and other dairy products such as yoghurt and ice cream…

Q: The PAM1 matrix penalizes mismatching amino acids more than higher numbered PAM matrices. Explain…

A: PAM (POINT ACCEPTED MATRIX) indicates the probability of a mutation of an amino acid in a given…

Q: 1) In MALDI-TOF data, when performing peptide mass mapping search, a) The number of peptides…

A: Protein identification with the help of mass spectrometry (MS) is an major step in proteomic…

Q: Electrophoresis 1.)a.) A protein required 6.8 min to travel 82 cm to the detector in a 96 cm -long…

A: Electrophoresis describes the separation of charged particles and their migration in the influence…

Microbial Taxonomy

Taxonomy is the science of naming, classifying, and describing organisms. The taxonomy will include all the plants, microorganisms, and animals on this planet. Carl Linnaeus coined the taxonomy term. He is known as the father of taxonomy. Microbial taxonomy is a criterion of grouping microorganisms into groups based on their similarities and past relatedness. Microorganisms belonging to the same group are differentiated from the others via grouping into categories. This differentiation makes the study of microorganisms easy. There are different types of microbial taxonomies like Numerical and Molecular taxonomy. The study of microorganisms is known as microbiology. Microbiology is a broad term that takes into account microbial cultures, taxonomic studies, evolutionary relationships. Microbial interaction and diversity are huge data that needs to be compiled under the subject of microbiology. Microbiology covers various organisms like bacteria, fungi, algae, plants, animals, etc.