A transcriptional regulator know as ComR may interact with a small protein, Prx 8.1 kDa). The complex of ComR-Prx was analyzed by gel filtration chromatography. A set of proteins were applied to Sephadex 200 Increase Superdex 200 (MW column (10 x 300 mm) at a flow rate of 0.75 mL/min in a 15 mM phosphate buffer, pH 7.4. The elution volumes of the proteins are summarized in the table below. Protein IgM Conalbumin Ovalbumin Chymotrypsinogen A Ribonuclease A MW (kDa) 970.0 75.0 44.0 25.6 13.7 Elution (mL) 9.5 13.9 15.0 16.6 17.5 a) Develop a selectivity curve from the protein set (standards). (Attach the curve and Excel sheet to the assignment) b) What is the exclusion limit of the resin (as determined from the selectivity curve)?

A transcriptional regulator know as ComR may interact with a small protein, Prx 8.1 kDa). The complex of ComR-Prx was analyzed by gel filtration chromatography. A set of proteins were applied to Sephadex 200 Increase Superdex 200 (MW column (10 x 300 mm) at a flow rate of 0.75 mL/min in a 15 mM phosphate buffer, pH 7.4. The elution volumes of the proteins are summarized in the table below. Protein IgM Conalbumin Ovalbumin Chymotrypsinogen A Ribonuclease A MW (kDa) 970.0 75.0 44.0 25.6 13.7 Elution (mL) 9.5 13.9 15.0 16.6 17.5 a) Develop a selectivity curve from the protein set (standards). (Attach the curve and Excel sheet to the assignment) b) What is the exclusion limit of the resin (as determined from the selectivity curve)?

Name: 3 A transcriptional regulator know as ComR may interact with a small p
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Description: 3 A transcriptional regulator know as ComR may interact with a small protein, Prx(MW = 8.1 kDa). The complex of ComR-Prx was analyzed by gel filtrationchromatography. A set of proteins were applied to Sephadex-200 Increase Superdex 200column (10 x 30

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter10: From Proteins To Phenotypes

Section: Chapter Questions

Problem 10QP: b. Compounds A, B, C, and D are known to be intermediates in the pathway for production of protein...

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