2. The mechanism of HMG-CoA reducatse enzyme activity involves several stages. For the catalyticreaction to proceed, another substrate known as NADPH, which acts as a reducing agent is required. Thekinetics of enzyme activity with NADPH at non-limiting amounts of HMG-CoA in the absence orpresence of compactin is shown below.a) Based on the description above, which of the sixtypes of enzymes does HMG-CoA reductase belongto?b) What is the Km for the reaction with no inhibitorpresent? Do NOT forget the units. Answers can beexpressed either as a fraction or in decimals.c) What happens the measured Km withincreasing amounts of compactin? (select one)increases decreases does not changed) What happens to the measured Vmax withincreasing amounts of compactin? (select one)increases decreases does not changee) Based on your answers above, what type ofinhibitor is compactin relative to its effects onenzyme activityNADPH?with-60-401/V, (nanomoles/min)-1-200.20.180.160.140.120.10.080.060.040.020€€Z +compact201/[NADPH], (MM)-1●None40+ 116 nM compactin60

For a one-substrate enzyme-catalyzed reaction, the Michaelis-Menton equation shows the quantitative…

Answered: 2. The mechanism of HMG-CoA reducatse…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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2. Draw the reactions catabolizing the amino acid valine into isobutryl-CoA, specifically one transamination reaction (for which you do not need to show the mechanism) and one dehydrogenation reaction (which is analogous to the pyruvate dehydrogenase reaction mechanism, and for which you need to show the mechanism using arrow pushing and including the relevant cofactors). Note: Beyond the importance of these reactions for human health, this pathway is used industrially in the production of isobutanol, an important biofuel. NH3 SCOA valine isobutyryl-CoA
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