18. Shown below are the results of a series of coinfections of E. coli B using T4 rlI- strains similar to those employed by Benzer in his deletion mapping experiment. Each strain contains a different deletion mutation. The ability to produce wild-type progeny phage is indicated by (+), (0) indicates no wild-type progeny. A D A B D E Which order of deletions is most consistent with this data? a. CDBEA b. DCEAB C. ABECD d. CDEAB e. EDCBA

18. Shown below are the results of a series of coinfections of E. coli B using T4 rlI- strains similar to those employed by Benzer in his deletion mapping experiment. Each strain contains a different deletion mutation. The ability to produce wild-type progeny phage is indicated by (+), (0) indicates no wild-type progeny. A D A B D E Which order of deletions is most consistent with this data? a. CDBEA b. DCEAB C. ABECD d. CDEAB e. EDCBA

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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A prototrophic strain (his" arg" lac') was used as a donor to transform an auxotrophic strain (his arg lac'). Initial transformants are isolated on minimal medium + histidine + arginine + lactose - glucose. i. What genotypes will grow on this medium? ii. These colonies are replicated to minimal medium + histidine, and 50% of the original colonies grow. What genotypes will grow on this medium? iii. The original colonies are also replicated to minimal medium + arginine, and 10% of the colonies grow. What genotypes will grow on this medium? iv. The original colonies are also replicated to minimal medium. No colonies grow. Based on this information, what genotypes will grow on minimal medium + histidine and on minimal medium + arginine? What is the relative gene order for his, arg, and lac? Which two genes are closer? Explain your answer.
A 2.0kb bacterial plasmid ‘BS1030’ is digested with the restriction endonuclease Sau3A; the plasmid map is depicted in the diagram below and the Sau3A (S) restriction sites are indicated. Which of the following DNA fragments do you expect to see on an agarose gel when you run Sau3A-digested plasmid ‘BS1030’ DNA? a. 250 bp, 450 bp, 550 bp, 1.1 kb, 1.5 kb and 2.0 kb b. 2.0kb c. 250 bp, 400 bp, 450 bp, 500 bp and 550 bp d. 100 bp, 200 bp, 250 bp, 400 bp, 500 bp and 550 bp
Recombinant protein production by a genetically modified Escherichia coli strain is proportional to cell growth. Ammonia is used as a nitrogen source for aerobic glucose respiration. The recombinant protein has the general formula CH1,55O0,31N0,25, while that of the cellular biomass is CH1,77O0,49N0,24. The biomass yield from glucose equals 0.50 g/g, while the recombinant protein yield from glucose corresponds to 20% of the cell yield from substrate.a) How much ammonia is required? What is the oxygen demand? (b) If the biomass yield remains the same, what are the ammonia and oxygen requirements for a wild-type strain of E. coli, with cell biomass of the same elemental composition, but unable to synthesize the recombinant protein? (c) On an industrial scale, cultivation takes place in a continuous fermenter at 28°C and the desired recombinant protein production rate is 7 g/h. Since the viscosity of the culture broth is considerable, the energy input due to agitation cannot be neglected.…
28. In a transformation experiment, DNA is collected from an E. coli donor strain of genotype cys(+) leu(-) thr(+) and used to transform a recipient of genotype cys(-) leu(+) thr(-). Initially, the treated recipient population is plated on a minimal medium supplemented with threonine. Many colonies are obtained. What are possible genotypes of these colonies? A. cys(-) leu(+) thr(+) B. cys(+) leu(+) thr(-) C. All are cys(+) and either + or - for leu and thr D. All are thr(+) and either + or - for cys and leu E. All are thr(-) cys(-) and either + or - - for CYS
An interrupted mating study is carried out on Hfr strains 1, 2, and 3. After conjugation is established, a small sample of the mixture is collected every minute for 20 minutes to determine the distance between the genes on the chromosome. Times of entry for each of the three Hfr strains studied in the interrupted mating are provided in parenthesis. The total duration of conjugation (in minutes) is given for each transferred gene. Hfr Strain Genes Transferred Hfr 1 oriT(0) met(2) ala(8) lac(13) gal(17) Hfr 2 oriT(0) met(2) leu(7) thr(10) azi(17) Hfr 3 oriT (0) gal(3) pro(8) trp(14) azi(19) Hfr 4 oriT leu met ala lac Hfr 5 oriT trp azi thr leu a. Use the data to construct a circular map of the bacterium.
We transformed E coli cells with a plasmid modified to contain a ‘virulence factor’ which would allow growth on media containing the antibiotic kanamycin (Kan). The plasmid confers constitutive resistance to ampicillin (Amp). Assume you were given competent cells of known transformation efficiency (TE). Assume TE= 1x10[7] (note 10[7] means 10 to the power of 7). You want to have about 1000 colonies on the P-200 plate. How many nanograms of plasmid should you use in the transformation reaction? Select only one answer. 1. 0.05 2. 20.00 3. 0.50 4. 50.00 5. 200.00 6. 5.00
1) Using the diagram below, sketch in the pattern of bands you would expect to see after digesting the DNA of the TT, Tt and tt genotypes of the TAS2R38 gene. Use the Base Pair Standards on the left of the diagram as a reference in drawing the positions of the bands. Base Pair TASTER GENO TYPE Standards (Base Pairs) TT Tt tt 500 400 300 200 100 50
In a transformation experiment involving a wild type bacterial strain with a recipient strain with mutations in genes f, g, h and i pairs of genes were analyzed for co-transformation with the following results: Gene Pair Co-transformation g+ i+ f+ i+ yes no f+ h+ Уes f+ g+ no g+ h+ h+ i+ no уes What is the linear order of these genes relative to cach other?
The genomes of two E. coli strains are compared in Figure14-19. Would you expect any third strain to contain moreof the blue, tan, or red regions shown in Figure 14-19?Explain.
We transformed E coli cells with a plasmid modified to contain a 'virulence factor' which would allow growth on media containing the antibiotic kanamycin (Kan). The plasmid confers constitutive resistance to ampicillin (Amp) Assume you were given competent cells of known transformation efficiency (TE). Assume TE= 1×10[6] (note 10[6] means 10 to the power of 6). You want to have about 1000 colonies on the P-200 plate. How many nanograms of plasmid should you use in the transtormation reaction? О 1.5.00 O 2.0.05 О 3.50.00 О 4.200.00 О 5.20.00 О 6.0.50
As stated in the text, bacteriophages have been discovered with the followingbase substitutions in their DNA:(a) dUMP completely substituting for dTMP(b) 5-hydroxymethyl-dUMP completely substituting for dTMP(c) 5-methyl-dCMP completely substituting for dCMPFor any one of these cases, formulate a set of virus-coded enzyme activitiesthat could lead to the observed substitution. Write a balanced equation foreach reaction you propose.
2c) If the whole potoroo genome is 4.2 x 10' bp, and the highlyrepetitive DNA in the potoroo genome is composed entirely ofcopies of the sequence 5'AAGACT' and its complement, howmany copies of this sequence are present in the potoroogenome?