14) The figure above is reproduced from Cole & Curtis' 1940 measurement of an action potential in the squid giant axon. Assume that these experiments were done at room temperature and the squid giant axon contains the following concentrations of Na+ and K+: Na+ inside: 10 mM, outside: 100 mM K+ inside: 300 mM, outside: 10 mM Is the measured action potential peak (110 mV) what you would expect to see? Why or why not?
Enzyme kinetics
In biochemistry, enzymes are proteins that act as biological catalysts. Catalysis is the addition of a catalyst to a chemical reaction to speed up the pace of the reaction. Catalysis can be categorized as either homogeneous or heterogeneous, depending on whether the catalysts are distributed in the same phase as that of the reactants. Enzymes are an essential part of the cell because, without them, many organic processes would slow down and thus will affect the processes that are important for cell survival and sustenance.
Regulation of Enzymes
A substance that acts as a catalyst to regulate the reaction rate in the living organism's metabolic pathways without itself getting altered is an enzyme. Most of the biological reactions and metabolic pathways in the living systems are carried out by enzymes. They are specific for their works and work in particular conditions. It maintains the best possible rate of reaction in the most stable state. The enzymes have distinct properties as they can proceed with the reaction in any direction, their particular binding sites, pH specificity, temperature specificity required in very few amounts.
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Fig. 3 Membrane action potential of the squid axon. The two horizontal traces are 50
millivolts apart. The resting potential in this axon was 58 millivolts. Thus the upper hori.
zontal line approximately represents zero potential difference across the membrane, the lower
line the resting potential (outside positive) and the action potential, starting from the resting
potential, swings to 110 millivolts (outside negative). Time intervals at the bottom are
0.2 misce.
14) The figure above is reproduced from Cole & Curtis' 1940 measurement of an action potential in the squid giant
axon. Assume that these experiments were done at room temperature and the squid giant axon contains the
following concentrations of Na+ and K+:
Na+ inside: 10 mM, outside: 100 mM
K+ inside: 300 mM, outside: 10 mM
Is the measured action potential peak (110 mV) what you would expect to see? Why or why not?
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- A resting axon's membrane potential is negatively charged.
- Voltage-gated ion channels open in response to stimulation, allowing positively charged ions into the cell and depolarizing the membrane potential.
- When the depolarization reaches a certain threshold, an action potential is generated and propagates down the axon.
- The membrane action potential refers to this depolarization and repolarization process that occurs along the axon.
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