1. If there was a negative charge at Ser 80 or Lys 84 which amino acid could be switched out and what probe sequence could then be used. Explain why that sequence should be used.

Q: Which of the above mutations will result in a shift in the reading frame

A: Mutations in a cell can be of several types: Chromosomal mutations - where one of the chromosomes in…

Q: 1.  List four general methods for experimentally inhibiting the activity of a specific gene with…

A: Gene inhibition is the regulation of gene expression in a cell that deactivates or silences or…

Q: 1. This complex assembles and organizes nucleosomes and contributes to gene repression Group of…

A: the answers to these multiple-choice questions are given below.

Q: 36.  When choosing restriction enzymes for cloning a gene of interest into a plasmid, what is an…

A: Restriction enzyme:           An enzyme that cuts dsDNA into fragments after recognising specific…

Q: segment shown in the diagram. The first three nucleotides of sequencing read 1 and sequencing read…

A: Nucleotides are the building blocks of DNA and RNA. They are organic molecules that consist of a…

Q: 12. You want to determine the location of the promoter and operator for an operon. To do so, you…

A: 1) If operator is removed, expression becomes constitutive 2) If promoter is removed, there should…

Q: 1. why does the genome contain so many more genes for rRNA than mRNA? 2. why is it effective for a…

A: Since you have asked multiple questions, we will solve the first question for you. If you want any…

Q: 1. An individual is heterozygous (CT) for a common C/T SNP that is tested using the Axiom microarray…

A: This question delves into the process of how an Axiom microarray, used by institutions like the UK…

Q: 4. Suppose you conduct the experiment and achieve the following results (assume adequate…

A: PSTI is Pancreatic Secretory Trypsin Inhibitor. It is also known as Serine protease inhibitor Kazal…

Q: coli WT (wild type, everything is functional) and II) E. coli whose lacl ger ed, when each of the 2…

A: the lacI gene is a regulatory gene for lac operon It produces a protein that blocks RNA polymerase…

Q: An RFLP can be created by any mutational event that cretes or deletes a restriction enzyme…

A: Introduction: In the field of molecular biology, restriction fragment length polymorphism (RFLP) is…

Q: What does the acronym CRISPR stand for?

A: CRISPR is a technique used in editing of base pair of genes. It is important tool in genetic…

Q: Is the BSA a good stadard to use in the bradford assay?

A: Introduction Proteins are the most abundant macromolecule present in our body. Proteins are made up…

Q: 11) Draw a bacterial expression vector with all required vector sequences. Be sure to label any…

A: Bacterial expression vectors are used to introduce foreign genetic material into a host in order to…

Q: 24. The diagram below shows the map for EcoRI restriction sites around the BRCA1 gene for two…

A: Answer well explained above.

Q: 5. Six colorectal cancer (CRC) patient samples and one normal colon biopsy from a healthy individual…

A: Patient 3, 4, 5 and 6 are exhibiting aberrant Wnt signalling when compared from normal.  Level of…

Q: I. In the lac operon, repression occurs when there is no lactose present. II. In the absence of…

A: When lactose absent and glucose is present no transcription of lac operon happens. Becaise lac…

Q: 3 The restriction endonuclease PstI cuts DNA symmetrically on both strands at the СTGCAG sequence:…

A: Restriction endonucleases are the enzymes that are used to form cuts inside the DNA molecule. It is…

Q: Based on the following wild type DNA sequence, indicate if each of the mutations should be…

A: A mutation is a change in a DNA sequence. Mutations can result from DNA copying mistakes made during…

Q: Expressing genes encoding a particular protein’s subunits from a plasmid in E. coli cells (volume of…

A: Escherichia coli (E. coli) are group of bacteria that are prevalent in the environment, foods, and…

Q: The 1000 aa protein (Protein X) you are studying has several putative caspase cleavage sites. You…

A: Western blotting is a molecular biology technique that is used to detect a specific protein from a…

Q: Here is the amino acid sequence of part of a hypothetical gene you want to clone:…

A: a.The amino acid sequence Tyr-Met-Cys-Trp-Ile will make a 14-mer probe with the least degeneracy as…

Q: Assume that a circular plasmid is 3200 base pairs in length and has restriction sites for HindIII…

A: HindIII is a Haemophilus influenzae type II site-specific deoxyribonuclease restriction enzyme that…

Q: gene editing and RNA interference.

A: Gene editing:  It is defined as the type of genetic engineering in which DNA is inserted or…

Q: 28. What type of mutation is seen here? WT: 5′-AUG GCU AGA GUU GAA AAA-3′…

A:

Q: the target DNA

A: CRISPR: It stands for Clustered Regularly Interspaced Short Palindromic Repeats. These are the…

Q: 29. siRNA involves in: Gene degenaration Gene downregulation

A: Small interfering RNAs (siRNAs) are 20- 27 base pairs long double-stranded RNA molecules. It is a…

Q: 7. Consider the 3 mechanisms of HGT in prokaryotes and identify 1) which one leads to the presence…

A: Horizontal gene transfer (HGT) refers to the transfer of genetic material from one species to…

Q: What is the natural purpose of restriction endonucleases in bacteria? O To digest the bacterial…

A: According to the question, we have to find out what is the natural purpose of restriction…

Q: 500 9. The restriction site sequence for the restriction enzyme Sau3AI and BamHI include four…

A: Introduction:- Restriction endonucleases are the bacterial enzymes that cuts double stranded DNA…

Q: 1.Knowing that the Control is 6.22 x 10- moles.channel-2sec-1 and the CFTR-CRISP is 3.84x 10-20…

A: The question is related to a gene therapy laboratory that is testing the effectiveness of a vector…

Q: Explain the Triplet Binding Assay ?

A: Introduction  In 1964, two eminent scientists Marshall W. Nirenberg and Philip Leder carried out the…

Q: 7. A 1000 bp EcoRI restriction fragment contains a gene you are interested in. The fragment was…

A:

Q: 6. Mutation analysis of GCK gene in patients with diabetes revealed a c.114 TàA (shown in bold and…

A: Restriction enzyme are this enzymes which can cut the DNA into fragments.

Q: pc*00ATAAADDATATAJOTTAA 1. Use the genetic code table and the information in the diagram below to…

A: Gene is a part of the DNA, located on a specific site on the chromosome and has a specific sequence…

Q: What item/s are true? 1. When the 3' -ATCGGCTAC-5' IS TRANSLATED THE COMPLEMENTARY STRAND…

A: DNA and RNA are important for cell function as DNA provide the information for the cell's…

Q: steps you would follow to generate a recombinant plasmid that can be used in downstream…

A: Plasmids are extra chromosomal material that exist independently of the main chromosome.  They may…

Q: D) organotroph 9. In the Ames test the number of histidine auxotroph colonies growing on media…

A: Mutation in biology is a change in the extra chromosomal genetic material, the virus, or the…

Q: fragments will be formed-a, b, and c. Which of the following gels produced by electrophoresis would…

A: A DNA segment is cut by two restriction enzymes and the three fragments are separated on an agarose…

Q: 8) Draw a two-stranded, antiparallel ß-sheet with at least four amino acids in each B-strand.

A:

Q: 1. Drag the red box to indicate the restriction site in the following sequence. 5-.. АТА ТСА TСС TGT…

A: Genetic engineering is one of main principles of biotechnology. It is a process of manipulation of…

Q: 4. A peptide was cleaved into smaller fragments using chymotrypsin and CnBr. The fragments were…

A: Introduction Peptides are peptide bonds that connect short chains of amino acids. Dipeptides,…