What is the purpose of the beta-galactosidase gene found in many plasmids for cloning?

Q: Which activity of E. coli DNA polymerase I is responsible for proofreading the newly synthesized…

A: The first known polymerase is the DNA Polymerase I. It is the enzyme that participates in…

Q: Why must the gene be inserted into a vector for it to be cloned?

A: Vectors are carriers of target DNA which are readily used in recombinant DNA technology. The most…

Q: What is the last step in metagenomics? Breaking open the bacterial cells Assembling short DNA…

A: Hello. Since your question has multiple parts, we will solve first question for you. If you want…

Q: you carry out DNA editing using CRISPR whter the editing template DNA has strands labeled with heavy…

A: DNA (Deoxyribonucleic acid) is edited with the help of several molecular tools. CRISPR (Clustered…

Q: PCR employs in vitro DNA replication. DNA replication in vivo-i.e., in a cell, normally requires…

A: Polymerase chain reaction (PCR) is a method widely used to make many copies of a specific DNA…

Q: 2. Your lab mate, Beaker, tells you that they have run into some issues with expressing a gene on a…

A: Lac operon: Lac operon is the system of gene expression by which bacterial cells can synthesise and…

Q: Plasmids are likely to contain

A: Plasmids are likely to contain:Antibiotic genes, A promoter associated with each gene, Restriction…

Q: 15. Open end: You get the plasmid form your collaborator. The plasmid contains the gene of the…

A: Plasmid vectors give a straightforward method of cloning little DNA fragments in…

Q: Priming for virus DNA replication can happen by all of the following EXCEPT: O a. Primase adding a…

A: Primer is a short nucleic acid, that is a starting point for DNA synthesis.

Q: Which of the following cloning strategy will be best to clone multiple DNA fragment (~10 fragments)…

A: Gibson assembly is a type of DNA assembly method that allows for the joining of multiple DNA…

Q: For Figure 15-7, draw out a series of steps that could explain the origin of this large plasmid…

A: We need to provide the process leading to the origin of a large plasmid containing various…

Q: arac PKAN-R 5,512 bp PARA 4,872 bp BamH PBAD Bam 377 bp kank ampR PBAD-rfp 807 bp Hindill Hindli

A: Answer :  The ristricton enzmye used to make cut in the plasmid.  Both the plasmid should be cut…

Q: is it possible that pcr primers could bind and amplify a sequence of dna that is difference from the…

A: Polymerase Chain Reaction (PCR) is a technique that gives us multiple copies of desired DNA…

Q: Most bacteria which are used in molecular biology are ampicillin sensitive and recA. Plasmid usually…

A: Mostly ampicillin sensitive and recA- bacteria are used in molecular biology for the transformation.…

Q: Im designing a restriction cloning experiment. My professor said that plasmid and insert DNA work…

A: It would have been simpler if the digested product had been visible at a lower point on the gel.…

Q: Select the definition of a microsatellite. short pieces of DNA surrounding the periphery of the…

A: Answer

Q: I the following statements about genetic exchange in bacteria is NOT true? a Plasmids do not have to…

A: Most bacteria are haploid organisms means carrying only one copy of each gene per cell which usually…

Q: WHAT is the subject of the figure above? WHAT is the main message of the figure? Give a BRIEF but…

A: Biotechnology is the use of our comprehension of biological processes to develop practical clinical…

Q: ori Clo fcoR v OFP Below is the plasmid map of pGLO which contains the Green Fluorescent Protein…

A: pGLO:   pGLO is an engineered plasmid that contains genes for beta- Lactamase.  Beta-lactamase…

Q: Assume the samples in this diagram of gel electrophoresis are DNA molecules. Why do the samples…

A: Gel electrophoresis is used to separate molecules of different sizes. It uses charged molecules that…

Q: Provide the SIGNIFICANT difference between the terms provided in the table below. Missense Nonsense…

A: Missense :  Missense mutation changes an amino acid to another amino acid.     Nonsense : Nonsense…

Q: .Describe the method you would use to test for the uptake of a plasmid with a DNA insert

A: Introduction: The introduction of recombinant DNA into a suitable host cell is followed by the…

Q: Which of the following best explains the natural function to the tumor-inducing (Ti) plasmid? It…

A: The deoxyribonucleic acid (DNA) is the double-stranded molecule that is the genetic material in most…

Q: 25. The restriction enzymes Kpnl and Acc65l recognize and cleave the same 6-bp sequence. You have a…

A: Restriction enzymes are the DNA digesting enzymes.

Q: Why does the probe bind to a specific site on a chromosome?

A: Cell division is the process by which a cell divides into two daughter cells. During this process,…

Q: 2. Restriction Enzyme Mapping - use any resources to assist you including the hints below. A…

A: Focus only on EcoR1.   Than focus on EcoR1 and HindIII.   Than focus on EcoR1, HindIII and Pst1

Q: For the plasmid below, list the origin and what antibiotic you would use for selection?

A: Plasmid is the extra chromosomal DNA present in prokaryotic cells. It contains information that…

Q: Match each description with the most appropriate term. Cuts apart foreign DNA as its primary…

A: There are several key terms and concepts in molecular biology that must be understood. This includes…

Q: . As described in the textbook, the plasmid vector pBluescript II contains an ampicillin resistance…

A: A plasmid is a single-stranded, circular DNA molecule that is separate from chromosomal DNA and may…

Q: Which of the following is a type of transposition that involves the movement of DNA within a genome…

A: DNA transposition is the movement of DNA sequence from one location to another. This movement is…

Q: Choose the false statement. O Genetic engineering includes recombinant DNA techniques whereby DNA is…

A: Genetic engineering is also known as the recombinant DNA technology.It involves the use of set of…

Q: Match the features of plasmids below with their meaning : Origin of replication LacZ gene antibiotic…

A: Plasmid is rounded Structure in the bacteria apart from main genome which functions as a a vehicle…

Q: explain topic is about how  recombinant DNA is  made

A: In this method DNA from two different species is isolated and inserted into a host organism to…

Q: Categorize the following as being associated with transformation, conjugation, or transduction. Note…

A: There are three principal methods of genetic transfer between bacteria. Transformation deals with…

Q: 2. You are planning to clone a gene into the PBR322 plasmid vector. The gene has Bgl II restriction…

A: Gene cloning is the technique to produce the exact copies of the desired gene by utilizing genetic…

Q: Supercoiling of DNA    requires GTP as source of energy   is only observed in prokaryotes   is…

A: DNA is a genetic material present in most living organisms. In case of eukaryotic cells DNA is found…

Q: Evaluate the paragraph below and drag the labels to complete the sentences that discuss antibiotic…

A: Antibiotic resistance is a growing worldwide problem that threatens public health. As bacteria…

Q: R plasmid R determinant CRISPR tracrRNA sgRNA E = A CRISPR nuclease that can cut foreign DNA and…

A: Molecular techniques are used to study the biological activities at a molecular level. These…

Q: You culture bacteria from the soil at a toxic waste dump on an agar plate and pick a single…

A: PCR, including Real-Time PCR which is probably the most widely used molecular technique especially…

Q: A number of advances have been made in biotechnology. CRISPR/Cas9 one of the most controversial, and…

A: CRISPR/Cas9 is found in the prokaryote system Cas 9 is an enzyme that cut DNA

Q: A target gene for producing human insulin must be inserted into a plasmid before the transformation…

A: Option 2 is correct only 1 and 3 follows

Q: ECOR1 cuts Plasmid T into 3 pieces. Feeling grumpy at your professor (☹), you decide to take a…

A: Introduction A plasmid is a little extrachromosomal DNA molecule that can replicate independently of…

Q: 39.  Which of the following are general features found in a plasmid used for bacterial cloning?…

A: Plasmids are cloning vectors in a cell that are autonomously replicating circular double stranded…

Q: Extra point question Antibiotic resistance can be acquired through many different mechanisms.…

A: Introduction In recombinant DNA technology, the antibiotic resistance principle is one of the prime…

Q: 52.Describe the basic processes of DNA clone by using E.coli 155 1202020155

A: The modification and manipulation of an organism's genes using technology is known as genetic…