39.  Which of the following are general features found in a plasmid used for bacterial cloning? Group of answer choices Origin of replication Multiple cloning site Multiple cloning site and antibiotic resistance gene Multiple cloning site, antibiotic resistance gene, and origin of replication Locus of transformation Locus of transformation, multiple cloning site, and antibiotic resistance gene Antibiotic resistance gene

Q: dye-linked substrate called X-gal (5-bromo-4-chloro-3-indolyl-ß-D-galacto-pyranoside) into galactose…

A: The lac operon contains a group of related genes (promoter, operator, Lac Z, Lac A, and lac Y) that…

Q: Where would the repressor be bound in an E. coli cell (nonmutant) that is NOT growing in lactose? O…

A: In E. coli, lactose is the substrate for the enzyme beta-galactosidase and it regulates the…

Q: Why must the gene be inserted into a vector for it to be cloned?

A: Vectors are carriers of target DNA which are readily used in recombinant DNA technology. The most…

Q: Which molecular processes are involved in Covid tests that use PCR? Gene expression Mutations C…

A: The SARS-CoV-2 virus, which causes COVID-19, has genetic material (ribonucleic acid or RNA), which…

Q: 36.  When choosing restriction enzymes for cloning a gene of interest into a plasmid, what is an…

A: Restriction enzyme:           An enzyme that cuts dsDNA into fragments after recognising specific…

Q: Which of the following cloning strategy will be best to clone multiple DNA fragment (~10 fragments)…

A: Gibson assembly is a type of DNA assembly method that allows for the joining of multiple DNA…

Q: Probe Loss of restriction site Insertion 4 Deletion> The smallest RFLP generated would be associated…

A: Introduction :  RFLP ( restriction fragment length polymorphism ) is an first generation molecular…

Q: Which of the following statements about bacterial genomes is NOT true? O a Each plasmid contains an…

A: Prokaryotes  In prokaryotes, membrane bound nucleus is absent. Number of chromosome is one. Mitosis…

Q: Site-directed mutagenesis involves: (MARK ALL THAT APPLY) Group of answer choices Joining two DNA…

A:

Q: This plasmid is digested with the enzymes listed below. Indicate how many fragments will be…

A:

Q: arac PKAN-R 5,512 bp PARA 4,872 bp BamH PBAD Bam 377 bp kank ampR PBAD-rfp 807 bp Hindill Hindli

A: Answer :  The ristricton enzmye used to make cut in the plasmid.  Both the plasmid should be cut…

Q: A commonly used plasmid that we will use in this lab is PUC19: (New England Biolabs) Indl 91 BamBI…

A: pUC19 is a circular double stranded DNA having 2686 base pairs.It is one of the most widely used…

Q: 24. The diagram below shows the map for EcoRI restriction sites around the BRCA1 gene for two…

A: Answer well explained above.

Q: What are the advantages of using a phage for cloning? Group of answer choices They allow for the…

A: Cloning is defined as a process where the scientists produce genetically identical copy of various…

Q: I the following statements about genetic exchange in bacteria is NOT true? a Plasmids do not have to…

A: Most bacteria are haploid organisms means carrying only one copy of each gene per cell which usually…

Q: 33) Judging by mosaic clone images above, what effect does loss of dmyc likely have on cell growth?…

A: Approach to solving the question:1. Observation of Mosaic Clone Images: Begin by carefully examining…

Q: 30) In the CRISPR system, the targeted DNA sequence is

A: CRISPR stands for clustered, regularly interspaced short palindromic repeats. Engineered CRISPR…

Q: What should the mRNA molecule of a regular mRNA vaccine contain to be translated accurately by our…

A: The correct answers are as follows- B. Poly A tail D. 5' cap E. Flanking UTRs F. Promoter The mRNA…

Q: pls help very urgent, no explaantion required, pls help asap

A: In molecular biology, a restriction digest is a technique for preparing DNA for analysis or further…

Q: ligated into plasmid vectors and transformed into bacterial cells/colonies on agar plates. This…

A: Answer is (D) Gene Library A gene library is a collection of total genomic DNA from an organism in…

Q: Technique whereby inserting DNA into a clone is accomplished using two different restriction enzymes

A: Directional cloning is the technique which is accomplished by cleaving the plasmid vector with two…

Q: Match the features of plasmids below with their meaning : Origin of replication LacZ gene antibiotic…

A: Plasmid is rounded Structure in the bacteria apart from main genome which functions as a a vehicle…

Q: Question: You conclude that all the blue colonies contain a plasmid

A: pUC is a plasmid cloning vector which is created by Joachim Messing and co-workers. Restriction…

Q: Important elements of a directed evolution experiment (“evolution in a test tube”) for an…

A: The goal of sciences is to create control over molecular structures and their reactivity. The…

Q: What is the purpose of the beta-galactosidase gene found in many plasmids for cloning?

A: For use as a selection marker in a food-grade cloning vector, the beta d galactosidase gene from…

Q: *Replication bubble and position of O. fragments. restriction enzyme rho-dependent termination

A: Asp per the guideline we are allowed to answer only starting 3. Please repost the rest.

Q: 36. Which is the sequence of steps used to amplify DNA with PCR during a single cycle? 1) Primers…

A: PCR is a technique that results in exponential amplification of a selected region of a DNA molecule…

Q: You clone the mouse FOXP2 gene into a plasmid vector, at a cloning site in the lacz gene. The…

A: Bacterial transformation involves introducing foreign DNA (in this case, the mouse FOXP2 gene) into…