
Concept explainers
Interpretation:
The explanation, with the help of a diagram, of the acetylation or phosphorylation of histones that could change their binding affinity with the DNA.
Concept Introduction:
Histones are the basic proteins that are found complexed with the DNA (deoxyribonucleic acid). They help in the packaging of the DNA, and their complex is known as chromatin. There are four types of histones named H2A, H2B, H3, and H4. Two molecules of each of these proteins are joined to form a histone octamer.
The histone octamer with the wrapped DNA is termed as nucleosome, and several nucleosomes connected by H1 histones form the chromatin. The string portion is termed as spacer region. There are about 150 base pairs of DNAs wrapped around one octamer, and the spacer or linking region contains about 30 to 50 base pairs.

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Chapter 9 Solutions
Biochemistry
- You’ve isolated a protein and determined that the Native molecular weight of the holoenzyme is 160 kD using size exclusion chromatography. Analysis of this protein using SDS-PAGE revealed 2 bands, one at 100 kD and one at 30 kD. The enzyme was found to be 0.829% NAD (by weight). What further can be said regarding the structure of the polypeptide?arrow_forwardWhat is the formation of glycosylated hemoglobin (the basis for the HbA1c test)? Can you describe it?arrow_forwardPlease analze the gel electrophoresis column of the VRK1 kinase (MW: 39.71 kDa). Also use a ruler to measure the length of the column in centimeters and calculate the MW of each band observed. Lane 1: buffer Lane 2 : Ladder Lane 3: Lysate Lane 4: Flowthrough Lane 5: Wash Lanes 6-8: E1, E2, E3 Lane 9: Dialyzed VRK1 Lane 10: LDHarrow_forward
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- 6. What is the IUPAC name of the following compound? A) (Z)-3,5,6-trimethyl-3,5-heptadiene B) (E)-2,3,5-trimethyl-1,4-heptadiene C) (E)-5-ethyl-2,3-dimethyl-1,5-hexadiene D) (Z)-5-ethyl-2,3-dimethyl-1,5-hexadiene E) (Z)-2,3,5-trimethyl-1,4-heptadienearrow_forwardConsider the reaction shown. CH2OH Ex. CH2 -OH CH2- Dihydroxyacetone phosphate glyceraldehyde 3-phosphate The standard free-energy change (AG) for this reaction is 7.53 kJ mol-¹. Calculate the free-energy change (AG) for this reaction at 298 K when [dihydroxyacetone phosphate] = 0.100 M and [glyceraldehyde 3-phosphate] = 0.00300 M. AG= kJ mol-1arrow_forwardIf the pH of gastric juice is 1.6, what is the amount of energy (AG) required for the transport of hydrogen ions from a cell (internal pH of 7.4) into the stomach lumen? Assume that the membrane potential across this membrane is -70.0 mV and the temperature is 37 °C. AG= kJ mol-1arrow_forward
- BiochemistryBiochemistryISBN:9781305961135Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougalPublisher:Cengage Learning
