Prescott's Microbiology
10th Edition
ISBN: 9781259281594
Author: Joanne Willey, Linda Sherwood Adjunt Professor Lecturer, Christopher J. Woolverton Professor
Publisher: McGraw-Hill Education
expand_more
expand_more
format_list_bulleted
Concept explainers
Videos
Textbook Question
Chapter 8.3, Problem 1RIA
Retrieve, Infer, Apply
1. What are depth filters and membrane filters, and how are they used to sterilize liquids?
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
Procedure Explant Preparation and Inoculation
1) The leaf explants were cut into squares (approximate size = 1cm x 1cm ).
2) The explants were soaked in 95% EtOH for 3 minutes with constant shaking.
3) EtOH was then decanted and 10% of Chlorox bleach was added together with a few drops of Tween 20, with constant shaking.
4) The explants were rinsed with autoclaved distilled water for at least 5 times.
5) The explants were then left to dry in the laminar air flow cabinet.
6) The explants were cultured in the appropriate media (2 explants in basal MS without BAP and the other two in MS with BAP).
7) Three explants were placed on its upper surface down and the other three with lower surface down in both basal MS media with and without BAP.
8) All the culture jars (name, date and treatments employed etc.)were labelled and incubated
them (in dark) in the growth chamber or culture room.
9) Explants were placed on the surface of the medium and the Petri dish were sealed with parafilm.
10)…
Citrate test
What critical enzyme allows the organism to acquire carbon from the medium?
bio 2 from Lab Manual 4 TH edition Leboffe and Pierce
Chapter 8 Solutions
Prescott's Microbiology
Ch. 8.1 - MICRO INQUIRY Which types of agents can be used...Ch. 8.1 - Retrieve, Infer, Apply 1. Define the following...Ch. 8.1 - Retrieve, Infer, Apply 2. What is the difference...Ch. 8.2 - MICRO INQUIRY Examine graph (a). How long would it...Ch. 8.3 - MICRO INQUIRY How might one verify that filtration...Ch. 8.3 - Retrieve, Infer, Apply 1. What are depth filters...Ch. 8.3 - Retrieve, Infer, Apply 2. Describe the operation...Ch. 8.3 - Retrieve, Infer, Apply 3. During the 20092010...Ch. 8.4 - Retrieve, Infer, Apply 1. Describe how an...Ch. 8.4 - Retrieve, Infer, Apply 2. In the past, spoiled...
Ch. 8.4 - Prob. 2.1RIACh. 8.4 - Retrieve, Infer, Apply 2. What is the correlation...Ch. 8.5 - MICRO INQUIRY Why is it important that all of...Ch. 8.5 - MICRO INQUIRY Why are cross-linking agents such as...Ch. 8.5 - Retrieve, Infer, Apply 1. Why are most...Ch. 8.5 - Retrieve, Infer, Apply Construct a table that...Ch. 8.5 - Retrieve, Infer, Apply 3. Which disinfectants or...Ch. 8.5 - Retrieve, Infer, Apply 4. How do phenolic agents...Ch. 8.5 - Retrieve, Infer, Apply 5. Which physical or...Ch. 8.6 - Retrieve, Infer, Apply 1. Briefly explain how the...Ch. 8.6 - Retrieve, Infer, Apply How does being in a biofilm...Ch. 8.6 - Retrieve, Infer, Apply Suppose hospital custodians...Ch. 8.6 - Retrieve, Infer, Apply Briefly describe the phenol...Ch. 8.6 - Retrieve, Infer, Apply Why might it be necessary...Ch. 8.7 - Retrieve, Infer, Apply How would you explain to a...Ch. 8.7 - Retrieve, Infer, Apply Propose the use of specific...Ch. 8 - Throughout history, spices have been used as...Ch. 8 - Design an experiment to determine whether an...Ch. 8 - Suppose you tested the effectiveness of...Ch. 8 - The death of Geobacillus stearothermophilus spores...
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- topic: gel electrophoresis What is the purpose of the running buffer?arrow_forwardDiscuss, how is it possible to isolate both aerobes and anaerobes using streak plates?arrow_forwardMicrotome 1. Define microtome instrument 2. Mention the types of microtome instrument 3. What are the principle operation of any type of microtome? 4. Compare between two typo of microtome difference from principle operationarrow_forward
- Periodic acid Schiff technique detail explanationarrow_forwardWhen preparing samples for SDS-PAGE, what is the purpose of boiling the samples at 95°C?arrow_forwardWhy should agar media be completely dissolved before they are dispensed in tubes and plates? What are the bases for pegging the temperature at 1210C for 15-30 minutes during moist heat sterilization and 1800C for two (2) hours using dry heat sterilization? Can you sterilize culture media using dry heat sterilization? Why is that so? You will notice in the videos shown, the cotton plug is not used. What is role of cotton plug in media prep, sterilization and culture of microorganisms? Instead of using cotton plug, plastic screw-cap is used, can you substitute this for the former? Is it technically acceptable in microbiology?arrow_forward
- selective media and explain its applications using specific prodving bacterial examples.arrow_forwardlist the stains and decolorizing agent used in acid fast staining method.arrow_forwardIsolating a pure culture from a mix sample is typically done on solid agar but can be performed using a liquid media. How can you isolate a pure culture from a mixed culture in a liquid medium (broth, not agar)?arrow_forward
- How does gel filtration work? What are the applications of analytical versus preparative versions of the method ?arrow_forwardWhat is the main difference between plates before and after centrifugation? What may be the purpose of concentrating the media and perform a second plating?arrow_forwardURGENTarrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
cell culture and growth media for Microbiology; Author: Scientist Cindy;https://www.youtube.com/watch?v=EjnQ3peWRek;License: Standard YouTube License, CC-BY