Prescott's Microbiology
10th Edition
ISBN: 9781259281594
Author: Joanne Willey, Linda Sherwood Adjunt Professor Lecturer, Christopher J. Woolverton Professor
Publisher: McGraw-Hill Education
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Chapter 8.4, Problem 1.1RIA
Retrieve, Infer, Apply
1. Describe how an autoclave works. What conditions are required for sterilization by moist heat? What three things must one do when operating an autoclave to help ensure success?
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Chapter 8 Solutions
Prescott's Microbiology
Ch. 8.1 - MICRO INQUIRY Which types of agents can be used...Ch. 8.1 - Retrieve, Infer, Apply 1. Define the following...Ch. 8.1 - Retrieve, Infer, Apply 2. What is the difference...Ch. 8.2 - MICRO INQUIRY Examine graph (a). How long would it...Ch. 8.3 - MICRO INQUIRY How might one verify that filtration...Ch. 8.3 - Retrieve, Infer, Apply 1. What are depth filters...Ch. 8.3 - Retrieve, Infer, Apply 2. Describe the operation...Ch. 8.3 - Retrieve, Infer, Apply 3. During the 20092010...Ch. 8.4 - Retrieve, Infer, Apply 1. Describe how an...Ch. 8.4 - Retrieve, Infer, Apply 2. In the past, spoiled...
Ch. 8.4 - Prob. 2.1RIACh. 8.4 - Retrieve, Infer, Apply 2. What is the correlation...Ch. 8.5 - MICRO INQUIRY Why is it important that all of...Ch. 8.5 - MICRO INQUIRY Why are cross-linking agents such as...Ch. 8.5 - Retrieve, Infer, Apply 1. Why are most...Ch. 8.5 - Retrieve, Infer, Apply Construct a table that...Ch. 8.5 - Retrieve, Infer, Apply 3. Which disinfectants or...Ch. 8.5 - Retrieve, Infer, Apply 4. How do phenolic agents...Ch. 8.5 - Retrieve, Infer, Apply 5. Which physical or...Ch. 8.6 - Retrieve, Infer, Apply 1. Briefly explain how the...Ch. 8.6 - Retrieve, Infer, Apply How does being in a biofilm...Ch. 8.6 - Retrieve, Infer, Apply Suppose hospital custodians...Ch. 8.6 - Retrieve, Infer, Apply Briefly describe the phenol...Ch. 8.6 - Retrieve, Infer, Apply Why might it be necessary...Ch. 8.7 - Retrieve, Infer, Apply How would you explain to a...Ch. 8.7 - Retrieve, Infer, Apply Propose the use of specific...Ch. 8 - Throughout history, spices have been used as...Ch. 8 - Design an experiment to determine whether an...Ch. 8 - Suppose you tested the effectiveness of...Ch. 8 - The death of Geobacillus stearothermophilus spores...
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- Procedure Explant Preparation and Inoculation 1) The leaf explants were cut into squares (approximate size = 1cm x 1cm ). 2) The explants were soaked in 95% EtOH for 3 minutes with constant shaking. 3) EtOH was then decanted and 10% of Chlorox bleach was added together with a few drops of Tween 20, with constant shaking. 4) The explants were rinsed with autoclaved distilled water for at least 5 times. 5) The explants were then left to dry in the laminar air flow cabinet. 6) The explants were cultured in the appropriate media (2 explants in basal MS without BAP and the other two in MS with BAP). 7) Three explants were placed on its upper surface down and the other three with lower surface down in both basal MS media with and without BAP. 8) All the culture jars (name, date and treatments employed etc.)were labelled and incubated them (in dark) in the growth chamber or culture room. 9) Explants were placed on the surface of the medium and the Petri dish were sealed with parafilm. 10)…arrow_forwardQuestion: Give the importance of biochemical test for the identification of the members of Enterobacteriaceae.arrow_forwardBiochemical and differential tests can be incorporated into growth media or performed on isolated colonies after they have been cultured. Give three examples of a differential test that is incorporated into media and three examples that are applied after the colonies have been cultured.arrow_forward
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