(a)
Interpretation:
Most polar phase should be identified from mobile phase and stationary phase in reverse phase column chromatography.
Concept Introduction :
Column chromatography is a large scale solid-liquid adsorption chromatographic technique that can separate multi-gram amounts of mixtures. This is a preparative version of thin layer chromatography that permits separation and isolation of products by collection of eluted fractions.
There are two types of column chromatography named as normal phase-column chromatography and reverse − phase column chromatography.
(b)
Interpretation:
Reverse-phase chromatography is more appropriate in separating peptides than normal-phase chromatography should be explained.
Concept Introduction :
Column chromatography is a large scale solid-liquid adsorption chromatographic technique that can separate multi-gram amounts of mixtures. This is a preparative version of thin layer chromatography that permits separation and isolation of products by collection of eluted fractions.
There are two types of column chromatography named as normal phase-column chromatography and reverse − phase column chromatography.
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Chapter 6 Solutions
EBK EXPERIMENTAL ORGANIC CHEMISTRY: A M
- Which of the following statements is incorrect? O A. In reversed-phase chromatography, the stationary phase is nonpolar. B. In reversed-phase chromatography, increasing the mobile-phase polarity increases the elution time. C. In reversed-phase chromatography, most polar component is eluted last. D. In normal-phase chromatography, the most polar component is eluted last. E. In normal-phase chromatography, the stationary phase is polar.arrow_forward7. In gas chromatography, increasing the column length leads to analytes, assuming all other parameters are kept constant. a. no effect on b. an increase in C. a decrease in 8. Suppose you are extracting an organic compound from an aqueous solution using retention time for dichloromethane. a. top layer b. bottom layer Once you mix the two layers together, which layer is the organic extract? 6001 9. Which way is best to remove an acidic impurity from an organic liquid that is immiscible with water? a. simple distillation b. wash with aqueous base c. wash with aqueous acid d. recrystallization it in diegolved in a volatilearrow_forwarda) What would you see if you ran a Thin Layer Chromatography (TLC) on a mixture of the three solids shown below using silica gel as the stationary phase and ethyl acetate/hexanes as the mobile phase? Whichsolid would have the highest Rf and which would have the lowest Rf? Explain b) Would changing the mobile phase (from part a) and increasing thepolarity by introducing small amount of methanol to the mobile phase change the order ofRf of the above 3 compounds? Explain c) Another type of chromatography is Reverse Phase Chromatography. In this type ofchromatography, the stationary phase is non-polar and the mobile phase is polar. If astudent performed Reverse Phase TLC on the mixture of 3 solids (from part a),what would be the order of Rf’s of the compounds? Which would have the lowest Rf andwhich would have the highest Rf?arrow_forward
- Differentiate normal phase chromatography from reverse phase chromatography. From which of the two governs the video demonstration in TLC and paper chromatography? How do you say so?arrow_forwardThin Layer Chromatography. All types of chromatography have mobile phaseand stationary phase. What is a stationary phase and a mobile phase? Elaborate your answer.arrow_forwardIn paper chromatography, a rectangular piece of filter paper serves as the mobile phase stationary phase eluent solute sample spotsarrow_forward
- Chromatography is a separation technique. What is the physical or chemical basis for separation of compounds during thin layer chromatography?arrow_forwardWhich of the following are necessary steps in performing paper chromatography? I. Saturate the chamber with the vapor of the mobile phase II. Allow the chromatogram to touch the sides of the beaker III. Place the spots at the same height as the solvents IV. Spot the samples 2 cm apart from each other a. I and IIIb. I and IVc. I, III and IVd. II, III and IVarrow_forwardWhich type of chromatography is paper chromatography? a.Column normal phase chromatography b.Planar reverse phase chromatography c.Column reverse phase chromatography d.Planar normal phase chromatographyarrow_forward
- What is the polarity of the compounds closest to the point of application on the filter paper when hexane is used as the mobile phase? What kind of chromatography is it according to the polarity of the stationary phase? Is hexane a suitable mobile phase for paper chromatography?arrow_forwardThe surface of the silica gel has a structure that looks like this diagram below. Suppose you used a plate coated with silica gel, with propanone, CH3COCH3, as the solvent for thin-layer chromatography. Suppose also that the mixture you were trying to identify contained benzaldehyde benzanilide phenol 1) rank the Rf value from large to small 2) name the IMF between each compound and the silica gel OH OH OH -0-Si-O-Si-O-Si-O- O main body of silica structurearrow_forwardMatch the terms with their definitions: 1. Adsorption chromatography 2. Partition chromatography 3. Ion-exchange chromatography 4. Molecular exclusion chromatography 5. Affinity chromatography A. Mobile-phase ions attracted to stationary phase ions. B. Solute attracted to specific groups attached to stationary phase. C. Solute equilibrates between mobile phase and surface of stationary phase. D. Solute equilibrates between mobile phase and stationary liquid film. E. Solute penetrates voids in stationary phase. Largest solutes eluted first.arrow_forward
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