(a)
Interpretation:
The Rf values of acetaminophen and caffeine should be determined.
Concept Introduction :
In chromatography, Rf value is the ratio between distance travelled by the component and distance travelled by the solvent front.
(b)
Interpretation:
The ingredients in a tablet of Midol should be determined based on the TLC analysis.
Concept Introduction :
Thin layer chromatography is a separation technique used for non-volatile substances. Depending on the affinity of the component of the mixture to the stationary phase and mobile phase, distance travelled by each component on the TLC plate differ.
(c)
Interpretation:
The mobile phase and stationary phase in the TLC experiment should be determined.
Concept Introduction :
All chromatography techniques have a stationary phase and a mobile phase. Stationary phase is a solid, or a liquid supported on a solid. Stationary phase is not migrating. The mobile phase flows through the stationary phase and carries components along with it.
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EBK EXPERIMENTAL ORGANIC CHEMISTRY: A M
- A chemist was able to extract a biologically active compound and wants to purify the sample since he is sure that the sample still contains impurities. After running the extract in TLC, the chemist found out that the sample produced only one spot near the solvent front. Suggest how the chemist can achieve a better resolution for the chromatogram.arrow_forwardYou are trying to separate a sample with 2 components: X and Y with GC. X has a lower boiling point than Y. Which one has a higher retention time? What is the most common way to increase retention of analytes in gas chromatography?arrow_forwardSFC combines important advantages of gas and liquid chromatography. It is especially useful for analyzing compounds which cannot be determined using either GC or LC. Qualify the statementarrow_forward
- A standard sample of compound A was placed on a silica TLC plate. After elution in 30/70 ethyl acetate/hexane, the sample showed a single spot at 3.4 cm (relative to the initial point) after the solvent had risen to 7.1 cm (relative to the initial point). A sample which was thought to contain this compound was analyzed by TLC and showed two spots after elution in the same solvent system. The solvent height for this plate was 6.5 cm (relative to the initial point) and the spots were located at 3.6 cm and 5.2 cm (relative to the initial point). Can either of these spots possibly be compound A? Explain. Include a discussion of the R, for each spot.arrow_forwardPaper Chromatography and TLC are examples of planar chromatography. True or FalsE?arrow_forward6. Say you are trying to separate two compounds by column chromatography. You run a column and collect the eluent in five fractions. You analyze each fraction by TLC on the same plate (shown below). Based on the TLC plate, how effective was your separation? -------1--- 1 2 3 4 5arrow_forward
- Consider the following errors that could be made when running TLC. Indicate what should be done to correct the error. a) A two-component mixture containing 1-octene and 1, 4-dimethylbenzene gave only one spot with an Rf value of 0.95. The solvent used was hexane. b) When a TLC plate was developed, the solvent front ran off the top of the plate.arrow_forwardWhich statement about TLC analyses is not true? * A- The line at the base of the TLC plate should be drawn with a pencil. B- To prevent contamination, a new capillary tube should be used to apply each sample. C- Samples should be applied multiple times in the same place to increase the concentrations of any possible compounds in the spot. D- A glass beaker lined with filter paper can be used as a development chamber in a TLC analysis. E- All these statements are correct.arrow_forward7. In gas chromatography, increasing the column length leads to analytes, assuming all other parameters are kept constant. a. no effect on b. an increase in C. a decrease in 8. Suppose you are extracting an organic compound from an aqueous solution using retention time for dichloromethane. a. top layer b. bottom layer Once you mix the two layers together, which layer is the organic extract? 6001 9. Which way is best to remove an acidic impurity from an organic liquid that is immiscible with water? a. simple distillation b. wash with aqueous base c. wash with aqueous acid d. recrystallization it in diegolved in a volatilearrow_forward
- 2arrow_forwardIn reversed phase HPLC, how will the retention time of a component of a mixture vary with the polarity of its molecules? Explain your answerarrow_forwardA solution was prepared by mixing 12.49 mg of B plus 10.00 mL of unknown containing A and just diluting to 25.00 mL. Peak areas of 5.97 and 6.38 were observed for A and B, respectively. Find the concentration of A (mg/L) in the unknown.arrow_forward
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