EBK EXPERIMENTAL ORGANIC CHEMISTRY: A M
6th Edition
ISBN: 9781305687875
Author: Gilbert
Publisher: CENGAGE LEARNING - CONSIGNMENT
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Chapter 6.2, Problem 3E
Interpretation Introduction
Interpretation:In a TLC experiment, the reason for the spot of the sample to be analyzed to not immersed in the solvent in the developing chamber should be explained.
Concept introduction:Thin Layer Chromatography is a sensitive chromatographic technique which used to analyze small quantities of the samples by solid-liquid adsorption method. Rectangular glass plate is coated with anadsorbent; sample to be analyzed is run on the plate in the developing chamber with the mobile phase to give spots of the analyzed sample.
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Chapter 6 Solutions
EBK EXPERIMENTAL ORGANIC CHEMISTRY: A M
Ch. 6.2 - Prob. 1ECh. 6.2 - Prob. 2ECh. 6.2 - Prob. 3ECh. 6.2 - Prob. 4ECh. 6.2 - Prob. 5ECh. 6.2 - Prob. 6ECh. 6.2 - Prob. 7ECh. 6.2 - Prob. 8ECh. 6.2 - Prob. 9ECh. 6.3 - Prob. 1E
Ch. 6.3 - Prob. 2ECh. 6.3 - Prob. 3ECh. 6.3 - Prob. 4ECh. 6.3 - Prob. 5ECh. 6.3 - Prob. 6ECh. 6.3 - Prob. 7ECh. 6.3 - Prob. 8ECh. 6.3 - Prob. 9ECh. 6.3 - Prob. 10ECh. 6.3 - Prob. 11ECh. 6.3 - Prob. 12ECh. 6.3 - Prob. 13ECh. 6.3 - Prob. 14ECh. 6.3 - Prob. 15ECh. 6.3 - Prob. 16ECh. 6.3 - Prob. 17ECh. 6.3 - Prob. 18ECh. 6.3 - Prob. 19ECh. 6.3 - Prob. 20ECh. 6.4 - Prob. 1ECh. 6.4 - Prob. 2ECh. 6.4 - Prob. 3ECh. 6.4 - Prob. 4ECh. 6.4 - Prob. 5E
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- how does TLC determine if a sample is pure?arrow_forwardIf a TLC was run in a solvent system that caused all the spots to show up at the baseline, how could the system be adjusted so that Rf values could be determined?arrow_forwardA student spots an unknown sample on a TLC plate and develops it in dichloromethane solvent. Only one spot, for which the Rf value is 0.95, is observed. Does this indicate that the unknown material is a pure compound? What can be done to verify the purity of the sample?arrow_forward
- In reverse phase chromatography which analyte will elute first, most polar or least polar?arrow_forwardThe identity and ratio of solvents are necessary in chromatography for achieving a good separation. What would happen if your solvent had an overly polar TLC developer? What would happen if it was overly non-polar?arrow_forwardDifferentiate between isocratic elution and gradient elution. Why is gradient elution sometimes a necessary technique in HPLC? What are the steps involved in gradient elution?arrow_forward
- What is the purpose of thin layer chromography lab? What is a hypothesis I can use when conducting a TLC lab and how can that hypothesis be tested?arrow_forwardWhy is it important to see the level of solvent in the developing chamber when running a TLC experiment.?arrow_forward5. Summarize your conclusions from the TLC simulator. Circle the correct answer in each description about TLC separation of organic compounds on silica gel plates. a) A more polar compound has a lower/higher retention factor. b) A less polar compound has a lower/higher retention factor. c) A more polar solvent causes a lower/higher retention factor. d) A less polar solvent causes a lower/higher retention factor. e) To increase the retention factor of a spot, you should add more ethyl acetate/hexane.arrow_forward
- What would happen if we use longer columns and select adsorbing materials with 200-400 mesh sizes for gel filtration chromatography using a column experiment?arrow_forwardPreparing a Thin Layer Chromatography experiment there are some common mistakes. Explain what happens in this situation: Sample spots too bigarrow_forwardThe three standards have different retention times on the Mini-GC. What physical characteristics of the molecules do you think contribute to this?arrow_forward
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